汞对小鼠生精细胞Bcl-2、Bax表达和生精细胞、精子超微结构的影响

Bcl-2 and Bax Expressions in Spermatogenic Cells and Ultrastructural Changes in Spermatogenic Cells and Sperms of Mice Exposed to Mercury

背景与目的:研究氯化汞暴露对小鼠睾丸生精细胞Bcl-2、Bax蛋白表达水平的影响,观察氯化汞引起生精细胞和精子超微结构的变化。材料与方法:取32只雄性ICR小鼠,随机分为3个不同氯化汞剂量处理组和阴性对照组,共4组,各处理组分别以0.5、1.0、5.0μmol/kg氯化汞腹腔注射,每天1次,连续5d,阴性对照组注射等体积生理盐水。用免疫组化法测定小鼠睾丸生精细胞Bcl-2、Bax蛋白表达的阳性细胞率、平均光密度值;透射电镜观察生精细胞和附睾精子超微结构改变。结果:3种不同剂量氯化汞组Bcl-2蛋白表达的阳性细胞率和平均光密度值均显著低于对照组(P<0.05),1.0、5.0μmol/kg氯化汞组Bax蛋白表达的阳性细胞率和平均光密度值均显著高于对照组(P<0.05)。透射电镜观察显示,各氯化汞处理组生精细胞核膜、染色质、线粒体、附睾精子线粒体等超微结构均发生不同程度的病理变化,且随氯化汞浓度的增加,超微结构病理变化越明显。结论:小鼠汞暴露可导致睾丸生精细胞Bcl-2、Bax蛋白表达阳性细胞率和平均光密度值显著性改变,生精细胞和精子超微结构发生明显的变化。

BACKGROUND ＆ AIM： To study Bcl-2 and Bax expressions in spermatogenic cells, ultrastructural changes in spermatogenic cells and sperms of mice after exposure to mercury. MATERIALS AND METHODS： 32 male mice were divided into 4 groups,3 groups treated with intraperitoneal mercuric chloride of 0.5, 1.0,5.0 μmol/（kg ·d） for 5 days, and one normal saline control group. The positive expression rates of Bcl-2 and Bax protein and average optical density in spermatogenic cells by the immunohistochemical method were assayed, and the ultrastructural changes of spermatogenic cells and sperms were assessed by the transmission electron microscope. RESULTS： Bcl-2 protein expression positive rates and average optical density were lower than of normal group（P 〈 0.05）.Bax protein expression positive rates and average optical density in 1.0, 5.0 μmol/kg groups were higher than those in normal group （P 〈0.05） .The ultrastructure of nuclear membrane, chromatin, mitochondria and sperm mitochondria in three treated groups showed different degrees of injury and the degree of uhrastructural change was more severe with increasing concentration of mercury. CONCLUSION： Mercury exposure caused abnormal Bcl-2 and Bax protein expression, average optical density change and ultrastructural changes in spermatogenic cells and sperms.