补肾益骨方对去势大鼠血生化、骨量和骨力学性能的影响

Effects of Bushen Yigu recipe(补肾益骨方) on biochemistry,bone quantity and biodynamics in ovariectomized rats

目的:观察补肾益骨方对去势大鼠血生化、骨量及其力学性能的影响;探讨补肾益气方能提高去势大鼠骨密度改善骨生物力学性能的机制。方法:取12周龄Wistar雌性大鼠40只,随机分为模型组(A)、治疗组(B)、阳性对照组(C)、正常对照组(D)4组,每组10只。D组行假手术(单纯剖腹后关闭),其余3组行完整双侧卵巢摘除术,术后常规饲养12周。A、D组以蒸馏水10ml/kg灌胃,B组以10ml/kg补肾益骨方提取液灌胃,C组以10ml/kgα-D3胶丸水溶液灌胃。用药12周后,取血清用电极法测定血清中Ca、P含量;用放免法测定血清中骨钙素(BGP)、降钙素(CT)水平;以双能X线密度测定仪测定骨密度;通过三点弯曲试验,记录载荷-变形曲线,测定骨生物力学指标。结果:治疗组血清中BGP、CT水平及骨矿含量、骨密度明显高于模型组,并改善了去势大鼠的骨生物力学性能。结论:补肾益骨方能提高去势大鼠骨密度、改善骨生物力学性能,推测激素BGP、CT水平变化是其重要的治疗机制之一。

Objective： To observe the effects of Bushen Yigu recipe on biochemistry, bone quantity and biodynamics in ovariectomized（OVX） rats and to explore its mechanism. Methods： Forty female Wistar rats were randomly divided into 4 groups, control group（ A）, treated group（ B）, positive control group（C） and normal control group（D）. The ovaries were excised in the group A,B,C,and the celiotomy was done in the group D（ no-excise ovaries）. Twelve weeks after the operation,group A, D were administed stilled water 10 ml per kilogram every day;group B were administed extract of Bushen Yigu recipe 10 ml per kilogram every day ; group C were administed water solution of α-D3 soft gelatin capsule 10 ml per kilogram every day. The rats were killed at 12 weeks after treatment, serum Ca, P level by electrode method, bone gla protein（BGP） and calcitionin （CT） by radioimmunity method, bone mineral content （ BMC ） by dual energy X-ray absorptiometry （ DEXA ）, were respectively determined. Bone biomechanical properties test were performed using a material testing machine. Results：Serum Ca, P, BGP, CT and BMC in the group B were obviously higher than group A. The biomechanical parameters in the group B were also improved. Conclusion ：Bushen Yigu recipe can increase BMC and obviously improve the bone biomechanical properties for OVX rats. The change of serum hormone may be the main mechanism in treatment of osteoporosis.