冷藏保护剂对坛紫菜幼苗冷藏效果的影响

Effects of cryoprotective agent on cold storage of Porphyra haitanensis larva

对采用不同冷藏保护剂保护的坛紫菜(Porphyra haitanensis)幼苗,经不同冷藏时间后的存活与生长发育情况进行研究,探讨影响冷藏效果的一些相关因素。结果显示:(1)在常温下,不同浓度梯度的胞内保护剂(甘油、DMSO)比胞外保护剂(葡萄糖、蔗糖)对坛紫菜幼苗成活的影响大,幼苗分别在20%DMSO和35%甘油中培养48 h,其成活率为15%和0%;(2)从单一冷藏保护剂的作用来看,甘油的冷冻保护效果优于DMSO,幼苗在20%-25%甘油保护剂作用下冷藏30 d,成活率为100%,冷藏90 d的成活率为25%-35%;葡萄糖和蔗糖作为单一的冷藏保护剂,冷藏15 d后幼苗全部死亡;(3)甘油+DMSO两种胞内保护剂混合冷藏30 d,成活率为85%;(4)甘油+DMSO+葡萄糖不同浓度组合的冷藏效果较好,冷藏180 d后仍达到95%以上的成活率;(5)盐度和藻体细胞壁的厚度与冷藏效果有关。当盐度为25时,冷藏藻体的细胞成活率高于其他盐度实验组;藻体细胞壁的厚薄对保存效果有影响,同一品系的藻体,细胞壁厚的比细胞壁薄的细胞更耐低温,成活率较高;(6)同株藻体不同部位冷藏30 d后成活率存在差异。

This article reports the cell survival and growth of Porphyra haitanensis after different cold storage time using different cryoprotectants, and discusses some correlative factors which may affect storage effect. Results are as follows： （ 1 ） In normal temperature, there was lower cdl survival of P. haitanensis at different concentrations of Glycerin and DMSO than that of Glucose and Sucrose. P. haitanensis were cultured in 20 % DMSO and 35 % Glycerin, and the survival rates were 15 % and 0 %, respectively. （2）Refrigerated in Glycerin, it had higher survival rate than at the same concentration of DMSO. Refrigerated in Glycerin for 30 d, the survival rate was 100 % ; refrigerated in Glycerin for 90 d, the survival rate was 25 % - 35 %. Refrigerated in Glucose and Sucrose for 15 d, the survival rate was 0%. （3）Refrigerated in combination of DMSO and Glycerin for 30 d, the survival rate was 85 %. （4）Different combination of DMSO, Glycerin and Glucose showed relatively good refrigeration effect. After 180 d refrigeration, the cell survival rate got to 95%. （5）Salinity and thickness of cell wall had some bearing on refrigeration effect. When salinity was 25, the cell viability was comparably higher than that at other salinity. The thickness of cell wall had some im- pact on the storage effect. In the same strain, the cells with thick cell wall possessed stronger low-temperature resistance and higher survival rate than those with thin cell wall refrigerated for 30 d. （6）In the different part of the same plant, some difference of survival rate also existed after refrigeration for 30 d. [Journal of Fishery Sciences of China,2007,14（3） ：450- 456]