红毛七超临界提取物化学成分的GC-MS分析

Analysis of supercritical fluid extracts of Radix caulophylli with gas chromatography-mass spectrometry

对红毛七超临界提取物进行分析。采用超临界二氧化碳流体萃取法(SFE-CO2)对秦巴山区特有药用植物红毛七进行提取。利用气相色谱/质谱联用技术(GC-MS)对提取物中的化学成分进行了分离分析,并采用峰面积归一化法计算各成分相对百分含量。对其中的49种化学成分进行了鉴定,所鉴定的成分占总流出峰面积的97.44%。其中脂肪酸类15种,占检出化合物总量的40.12%;酯类6种,占28.84%;烯类4种,占总11.30%;醇类12种,占5.92%;酮类化合物6种,占4.74%;生物碱类3种,占2.61%。实验结果为了解红毛七的化学物质基础和进一步开研究提供了依据。

To analyze the constituents in supercritical fluid CO2 extraction （SFE-CO2 ） of Radix caulophylli, the Radix caulophylli was extracted with SFE-CO2,and analyzed by gas chromatography-mass spectrometry （GC-MS）. The GC-MS analysis with a DB-5MS capillary column （30 mm × 0. 32 mm ID, 0.25μm film thickness） was used. The inlet temperature was maintained at 280 ℃. The column oven was held at 80 ℃ for 2 min, then programmed from 80 to 280 ℃ at 5 ℃ · min^-1 and, finally, held for 4 min. Helium at a constant flow rate of 2.0 mL·min^-1 was used as the carrier gas. The mass spectrometry conditions were as follows： ionization energy, 70 eV; ion source temperature, 200℃. The mass selective detector was operated in the TIC mode （m/z was from 40 - 500）. For the first time 49 peaks were separated and identified, the compounds were quantitatively determined by normalization method, and the identified compounds represent 97. 44% of total GC peak areas. Viz, n-hexadecanoic acid （31.4%）, （ E, E ） -9, 12-octadecadienoic acid （ 26.54% ）, （ Z ）-7-tetradecenal （ 9.4% ）, hexadecenoic acid （3.23%）, 10-undecenal （3.22%）, octadecanoic acid （2.25%）, and caulophylline （ 1.76% ） etc. The results will provide important foundation for understanding the constituents and further exploitation of Radix caulophylli.