丹参和天麻素在大鼠脑出血后的治疗作用

Therapeutical Effect of Salvia miltiorrhiza and Gastrodin to Rats after Cerebral Hemorrhage

目的:探讨脑出血血肿周围细胞免疫机制及丹参、天麻素的治疗作用。方法:用免疫组织化学及组织HE方法,观察40只大鼠尾壳核脑出血后3、7 d及药物治疗组的血肿周围区域CD3+、CD8+淋巴细胞的表达和微血管增生情况。结果:脑出血后血肿周围存在CD3+、CD8+淋巴细胞表达。在脑出血3 d时丹参和天麻治疗组血肿周围CD3+淋巴细胞数明显高于对照组,CD8+淋巴细胞数低于对照组。丹参组在此期间血管增生明显。出血7 d后丹参治疗组血肿周围CD3+淋巴细胞数明显高于对照组,CD8+淋巴细胞数低于对照组;而天麻组CD3+淋巴细胞数下降,但血管增生显著。结论:丹参和天麻素在脑出血治疗过程中均表现出免疫调节和血管增生作用。

Objective： To study the immunological mechanism of peri-haematoma cells after cerebral hemorrhage and the therapeutal effect of raix Salviae miltiorrhizae （RSM） and gastrodin. Methods： Immunohistochemical and histological techniques were used to detect CD3 ^＋ lymphocytes and CD8 ^＋ lymphocytes in peri-haematoma area of dorsal caudate putamen of 40 experimental rats on the 3^rd and 7^th days offer cerebral hemorrhage. The number of peri-haematoma microvessels was observed as well. RSM and gastroin were intraperitoneally injecfied to rats in RSM treatment group （RG） and gastroin treatment group （GG） separately in 2 hours after the experimental cerebral hemorrhage. Results： CD3 ^＋ lymphocytes and CD8 ^＋ lymphocytes appeared in tissue around the hematoma. CD3 ^＋ lymphocyte amount increased and CD8 ^＋ lymphocyte amount decreased in RG group and GG group on the 3^rd day, compared to those of control group. The number of micro-vessels was remarkably increased in RG group. On the 7^th day, higher CD3 ^＋ lymphocytes and lower CD8 ^＋ lymphocytes were still appeared in RG group, but CD3 ^＋ lymphocyte decreased notablely in GG group. Significant proliferation of microvessels occurred in GG group. Conclusion： RSM and gastodin are both effective in immunoregulation and in promoting of micro-vessels proliferation during the process of cerebral hemorrhage treating.