海洛因依赖大鼠胰岛D细胞免疫组织化学研究

Immunohistochemical Study on D Cells in Panceatic Islet of Heroin Dependent Rats

目的:探讨海洛因依赖对大鼠胰岛生长抑素(somatostatin,SS)免疫反应(IR)细胞(D细胞)的影响。方法:用免疫组织化学SABC单染法、形态计量法,观察海洛因依赖大鼠胰岛SS-IR细胞免疫组织化学反应及平均灰度值的变化。结果:与正常组和盐水对照组比较,海洛因依赖大鼠胰岛SS-IR细胞免疫染色增强,平均灰度值降低,差异有显著性。结论:胰岛SS-IR细胞参与了海洛因依赖期间机体的调节过程。

Objective： The morphological and functional changes of somatostatin immunoreactive（ SS- IR） ceils （D cells） in pancreatic islets of rats were studied to investigate the effect of heroin dependence on the secretion of SS- IR cells. Methods ： The heroin dependent model of rats was made by subcutaneous injection of heroin. The immunohistochemical SABC staining method and image analysis were adopted in the studies. Results： The immunological staining was obviously stronger, and mean grey degree was remarkably lower in SS-IR cells of rats in heroin dependent group than that in rats of normal control group. Conclusions： By decreasing the secretion of SS, the SS-IRceils of islet involved in body regulation during the heroin dependence process.