瘦素及胰岛素样生长因子-I与卵泡发育的关系

The Relationship of Levels of Insulin-like Growth Factor-I and Leptin with the Development of Ovarian Follicles

目的:探讨卵泡液和血清中瘦素(Leptin)与胰岛素样生长因子-I(IGF-I)水平与卵泡发育的关系。方法:应用放射免疫法(R IA)检测67例患者取卵日血清及卵泡液中的Leptin与IGF-I水平。结果:(1)取卵日血清中的Leptin水平为(13.20±5.43)mg/L,显著高于卵泡液中Leptin水平(10.86±5.73)mg/L,P<0.05;卵泡液中的Leptin水平与血清中的Leptin水平呈正相关(r=0.84,P<0.001)。血清中的IGF-I水平为(7.18±4.58)mg/L,显著高于卵泡液中IGF-I水平(4.36±3.03)mg/L(P<0.05),卵泡液中的IGF-I水平与血清中的IGF-I水平呈正相关(r=0.79,P<0.001);(2)卵巢低反应型者的血清及卵泡液中Leptin水平显著高于卵巢中高反应型者,差异有显著性(P<0.05);(3)三种卵巢反应类型的血清及卵泡液中IGF-I水平比较,差异无显著性(P>0.05);(4)卵泡液及血清中的Leptin水平与IGF-I水平无明显的相关性(r=-0.079,P>0.05)及(r=-0.127,P>0.05)。结论:(1)卵泡液中的Leptin及IGF-I均来自血清;(2)高浓度的Leptin可能抑制卵泡发育,而低浓度的Leptin可能对卵泡发育没有影响;(3)体内IGF-I可能与其受体结合后发挥对卵泡发育的调节作用;(4)Leptin与IGF-I之间无明显相关关系。

Objective： To investigate the relationship of the levels of insulin-like growth factor I （IGF-I） and leptin with the development of ovarian follicles. Methods： Radioimmunoassay was used to determine levels of IGF-I and leptin in serum and follicular fluid obtained during oocyte picking-up （OPU） in 67 cases of in vitro fertilization and embryo transfer （IVF-Et）. Results： （1） Leptin levels in serum were significantly higher than those in matched follicular fluid, [ （13.20 ± 5.43 ）ng/ml and （10.86 ±5.73 ）ng/ml respectively （P 〈0.05）], and were positively correlated with those in follicular fluid（ r =0.84, P 〈0.001 ）. IGF-I levels in serum were significantly higher than those in matched follicular fluid [ （7.18 ± 4.58 ） ng/ml and（4.36± 3.03 ） ng/ml, respectively, （ P 〈 0.05 ） ], and were positively correlated with those in follicular fluid（r =0.84 P 〈0. 001 ）. （2） Leptin levels in serum and follicular fluid of patients with lower ovarian reaction were significantly higher than those of patients with middle and higher ovarian reaction （ P 〈 0.05 ）. （3） There was no significant difference of IGF-I levels in serum and follicular fluid among patients with different ovarian reaction （ P 〉 0. 05 ）. （4） There was no significant correlation between leptin and IGF-I levels in serum and follicular fluid（ r = - 0. 079 P 〉 0. 05 ） 及（ r = - 0. 127 P 〉 0.05 ）. Conclusions： （ 1 ） IGF-I and leptin in follicular fluid come from serum; （2） Higher concentrations of leptin may restrain follicular development, while lower concentrations of leptin may not influence follicular development; （3） The regulation of IGF-I to follicular development is possibly educed after the combination of IGF-I with its receptor; （4） There is no significant correlation between leotin and IGF-I.