胰岛素样生长因子结合蛋白-4在食管鳞癌中的表达及临床意义

Clinical significance of insulin-like growth factor binding protein-4 expression in esophageal squamous cell carcinoma

目的探讨胰岛素样生长因子结合蛋白-4（IGFBP-4）在食管鳞癌中的表达及其临床意义。方法应用RT—PCR及免疫组化方法检测23例食管鳞癌及正常组织IGFBP-4、基质金属蛋白酶-7（MMP-7）的表达情况。结果食管鳞癌IGFBP-4 mRNA表达高于正常组织（P〈0．01），但在肿瘤侵及外膜以上及有淋巴结转移者表达降低（P〈0．01），在不同年龄、性别及肿瘤大小间表达差异无统计学意义（P〉0．05）。癌组织IGFBP-4蛋白表达阳性率为60．9％，低于正常组织（91．3％）（P〈0．05）。MMP-7 mRNA在食管鳞癌组织的表达为100．0％，正常组织未见表达（P〈0．01），肿瘤侵及外膜以上及有淋巴结转移组表达上调更明显（P〈0．05）。IGFBP-4与MMP-7 mRNA表达呈负相关（P〈0．01）。结论IGFBP-4表达下调与食管鳞癌的浸润及转移有密切关系，并与MMP-7高表达负相关，IGFBP-4 mRNA检测对判断食管癌进展及预后有重要意义。

Objective To investigate clinical significance of insulin-like growth factor binding protein-4 expression in esophageal squamous cell carcinoma. Methods The expression of IGFBP-4 and MMP-7 in tumor and normal tissues were determined by RT-PCR and immunohistochemical method in 23 esophageal squamous cell carcinoma. Results The expression of IGFBP-4 mRNA was higher in esophageal cancer tissues than normal tissues（ P 〈 0. 01 ） . But much lower expression of IGFBP-4 mRNA was found in tumor tissues with senosa infiltration and lymph node metastasis （P 〈 0. 01 ）. There was no significant difference between ages and sex and tumor size （P 〉 0. 05）. The positive rate of immunohistochemical expression of IGFBP-4 in tumor tissues was lower than that in control group （60. 9% vs 91.3%, P 〈0. 05 ） . The expression level of MMP-7 mRNA was higher in esophageal cancer tissues than that in normal tissues （P 〈0.01 ）, and it showed a correlation with the depth of infiltration and lymph node metastasis. The expression of IGFBP-4 mRNA was negatively correlated with that of MMP-7 （ P 〈 0. 01 ）. Conclusion The lower expression of IGFBP-4 mRNA was related to the depth of infiltration and lymph node metastasis, and it was negatively correlated with the expression of MMP-7 mRNA. These data indicated that it may be benefit to evaluate the advancement and prognosis of esophageal cancer by investigating the expression of IGFBP-4 mRNA