实验性脑积水蛛网膜绒毛内皮细胞CD31和vWF表达研究

Expressions of CD31 and vWF in Arachnoid Villus Endothelium Cell from Experimental Animal with Hydrocephalus

目的了解脑积水不同时段动物蛛网膜绒毛内皮细胞CD31、vWF因子变化情况。方法通过经枕大池-四脑室内注射入硅油的方法,建立犬脑积水模型。观察术后实验犬行为学改变,根据改良Tarlov评分标准对犬神经功能进行评分。各实验组动物分别于术后3d(急性期)、2周(亚急性期)及12周(慢性期)时行磁共振检查了解脑积水形成情况并进行脑室测量。并通过免疫萤光组织化学实验观察不同时期脑积水犬蛛网膜绒毛内皮细胞的CD31,vWF因子的表达。结果四脑室注射硅油法建立犬脑积水模型成功。注射后实验犬出现脑积水症状,Tarlov评分下降,磁共振影像显示脑室进行性扩大。在整个静脉窦内皮细胞上,都显示CD31阳性,包括蛛网膜绒毛表面的内皮细胞,其显色强度与窦内皮细胞无异。蛛网膜绒毛内皮细胞CD31因子的表达在脑积水各组与正常对照组之间没有差异。在假手术对照组,蛛网膜绒毛顶端内皮细胞的vWF的表达较窦壁内皮细胞明显减弱。在脑积水急性期,vWF表达的增加尚不明显;但随着脑积水的进展,亚急性组、慢性组蛛网膜绒毛顶端内皮细胞的vWF的表达明显增加。结论四脑室注射硅油法可以建立模拟临床的犬脑积水模型。实验动物脑积水进展期蛛网膜绒毛顶端内皮细胞vWF的变化可以提示蛛网膜绒毛吸收脑脊液的功能改变。

Objective The aim of this study was to investigate the CD-31 and vonWillebrand factor （vWF） expressions and the cell morphological alterations ofarachnoid villus endothelium in different stages of animal hydrocephalus. Methods Silicon oil was injected into the fourth ventricle of experimental canine for buildinghydrocephalus model. The post-injection ethology of the canine was observed, and thescoring of Tarlov was performed. The magnetic resonance （MR） images of canineskulls were achieved for verification of hydrocephalus at 3 day, 2 weeks, and 12 Cweeks post-injection. The expressions of CD-31 and yon Willebrand factor （vWF） inthe arachnoid villus endothelium cells at different stages of hydrocephalus werestudied by immunofluorescence histochemistry. Results Experimental hydrocephalusanimal model was built up satisfactorily by injection of silicon oil. Experimentalcanines appeared the hydrocephalus symptoms and Tarlov scoring decrease. MRimages revealed progressive ventricular enlargement in different stages ofhydrocephalus. The immunofluorescence histochemistry staining showed that CD31 expressed positively in all the endothelium cells, including the endothelium cell of thevillus. The immunofluorescence histochemistry staining showed that the vWFexpression in the endothelium of the villus was diminished when compared to that innormal sinus wall. And in sub-acute and chronic hydrocephalus, the expression of vonWillebrand factor increased. Conclusions The fourth ventricular injection of siliconoil may induce the hydrocephalus animal model. The expression of yon Willebrandfactor in the endothelium cell of arachnoid villus increases during progressedhydrocephalus.