角质细胞生长因子及其受体在Hela细胞中的表达及其作用

Expression of Keratinocyte Growth Factor and Keratinocyte Growth Factor Receptor and Its Impact on Hela Cells

目的确定角质细胞生长因子(KGF)及其受体(KGFR)在Hela细胞中的表达及KGF对Hela细胞增殖的影响。方法取对数增长期的Hela细胞,采用RT-PCR在基因水平确定KGF及KGFR在Hela细胞中的表达;Western blot及ELISA在蛋白水平确定KGF及KGFR在Hela细胞中的表达;3H-TdR掺入试验测定KGF对Hela细胞增殖的影响。结果①从基因水平和蛋白水平证实KGF及KGFR在Hela细胞中有表达。②人重组KGF因子对Hela细胞有明显的增殖作用。③用KGF抗体中和Hela细胞自分泌的KGF后,Hela细胞的增殖与对照组相比减弱(P<0.05)。结论Hela细胞中有KGF和KGFR的表达;KGF对Hela细胞有明显的增殖作用。

Objective To identify the expression of keratinocyte growth factor （KGF） and keratinocyte growth factor receptor （KGFR） in Hela cells and the impact of keratinocyte growth factor on Hela cells. Methods Reverse transcriptase polymerase chainreaction （RT-PCR） method was employed to determine the gene expression of KGF and KGFR in Hela cells. The ELISA and Western blot methods were employed to determine the protein expression of the KGF and KGFR. The ^3H-Thymidine incorporation method was employed to determine the impact of the KGF on the proliferation of Hela cells. Results The KGF and KGFR genes were expressed in the Hela cells. The KGF and KGFR proteins were expressed in the Hela cells. The Hela cells were stimulated to proliferate by the recombinant human KGF. The proliferation of the autocrine KGF in the Hela cells was neutralized by the KGF antibodies significantly （Dunnett＇s test, P〈0. 05）. Conclusion KGF and KGFR are expressed in Hela cells. Both autocrine and recombinant human KGF has an impact on the proliferation of Hela cells.