CO_2和5%葡萄糖溶液作为膨宫介质对子宫内膜癌细胞增殖和凋亡的影响研究

Effect of 100% CO_2 or 5% Dextrose as Distending Uterine Cavity Medium on the Proliferation and Apoptosis of Endometrial Carcinoma Cell in vitro

目的探讨CO2和5%葡萄糖溶液哪一种更适合作为疑似子宫内膜癌患者宫腔镜操作的膨宫介质。方法实验分为5%葡萄糖溶液组、CO2组和对照组。将人子宫内膜癌细胞(HEC-1-B)分别放置于100%CO2气体、4.00kPa灌注压、37℃环境下和5%葡萄糖溶液、37℃环境下1h,对照组无特殊处理。连续9d每隔24h,采用MTT法检测各组子宫内膜癌细胞增殖的变化;分别在处理后第1、3、5、7、9d,采用流式细胞仪碘化丙啶(PI)活细胞染色检测细胞周期G1期的变化。结果对照组和CO2组子宫内膜癌细胞A490值的上升趋势较5%葡萄糖溶液组明显,差异有统计学意义(P<0.05);而对照组和CO2组之间比较,第1～6d均是对照组A490值略高于CO2组,但除第5d外,差异均无统计学意义(P>0.05);第7～9d,对照组A490值略低于CO2组,差异也无统计学意义(P>0.05)。对照组、5%葡萄糖溶液组、CO2组G1峰值均呈逐渐上升趋势,5%葡萄糖溶液组G1峰值的上升趋势较对照组和CO2组明显,差异有统计学意义(P<0.05)。而对照组和CO2组之间比较,第1、3、9d均是对照组G1峰值略高于CO2组,第5、7d,对照组G1峰值低于CO2组,但差异无统计学意义(P>0.05)。结论CO2和5%葡萄糖溶液比较,5%葡萄糖溶液抑制子宫内膜癌细胞增殖的作用较CO2强,促进子宫内膜癌细胞凋亡的作用也较CO2强。

Objective To study the effect of 100% CO2 or 5% dextrose on proliferation and apoptosls of endometrial cancer ceil （HEC-1-B）, and to find out which one is the better distending uterus medium available to patients suspected to have endometrlal cancer. Methods Endometrial cancer cells were divided into 3 groups： control group; 5% dextrose group; 100% CO2 group. In the 5% dextrose group, cells were put in 37℃ and 5% dextrose environment for 1 h. In the CO2 group, cells were put in 37℃ and 100% CO2 with 30 mmHg environment for 1 h. The control group was not administrated with any thing but for 37℃ and 5% CO2. The optical density （A490） value of endometrlal cancer cells were measured with MTT chromometry every 24 hours within next 9 days. The G1 peak values of endometrial cancer cells were detected with PI staining and flow cytometry method on 1st, 3rd, 5th, 7th, 9th day after cell treated. All data were analyzed by SPSS 11. 5 software. Results The A490 values of the three groups all tended to rise, but the levels of control group and CO2 group were more significantly high than 5% dextrose group （P〈0. 05）. No significant difference of the A490 value was observed within next 9 days between the control group and CO2 group. The G1 peak values of the three groups all had the tendency to rise. In the 5% dextrose group, the G1 peak value was significantly increased comparing with the control group and CO2 group （P〈0. 05）. No significant difference of the G1 peak value was observed between the control group and CO2 group （P〈0.05）. promote the apoptosis of the endometrial cancer cells. endometrlal cancer cells. Conclusion 5% dextrose can inhibit the proliferation and CO2 has no effect on the proliferation and apoptosis of the