急性绞窄性肠梗阻肠道细菌移位实验观察

Translocation of Indigenous Bacteria from Gastrointestinal Tract to Internal Viscera in Acute Strangulated Intestinal Obstruction

本文通过36只小白鼠的急性绞窄性小肠梗阻动物模型观察肠道定植细菌移位到系膜淋巴结及其他器官(肝、脾、心、肺及腹膜腔)。结果发现:梗阻15min,系膜淋巴结等器官细菌检出率达50％以上,2h后,腹膜腔渗液和肝脏细菌培养全部阳性。移位的菌群主要是大肠杆菌、脆弱拟杆菌群、肠球菌和厌氧球菌等,与临床内源性感染菌群相似。梗阻发生15,30,60,90,120min,后需氧菌移位的机率无显著性差异(P>0.05),而厌氧菌则以15,60,120min组检出较高。脏器以系膜淋巴结捡出最高,其次是肝、脾及腹膜腔。

Observation on the intestinal bacteria translocation to a number of internal viscera in 36 mice suffered from acute strangulated intestinal obstruction. Qualitative bacteriology studies were made at 15rain to 2 hours time intervals on peritoneal fluid, mesenteric lymph nodes (MLN)liver, pleen, heart blood, and Lung. At 15min period, more than 50％ of the specimens were cultured positive, whereas at 2 hours preiod 100％ of the MLN and liver were cultured positive. Strains of bacteria identified predominantly were that of E. coli, B, fragilis group, Enterococcus and some anaergbi cocci. No difference was obsrved for the aerobes whe- reas for the anaerobes, translocation was observed to be definitely bighter in the 15, 60 and-120min, than that in the 30 and 90min groups. MLN listed highest in incidence, followed by liver, spleen and peritoneal fluid.