HPLC法测定多维片中的水溶性维生素

Determination of Water Soluble Vitamins in Drug by HPLC

研究药品中水溶性维生素含量的高效液相色谱测定方法。色谱柱：Micropak MCH-10;紫外检测器波长：254 nm;流速：1 mL.min^-1。柱温：40℃;用磷酸调整pH;色谱条件：先采用pH值为3.0的20%甲醇流动相淋洗,3分钟后改用pH为6.0的40%甲醇流动相淋洗。维生素C、维生素B2和烟酰胺的线性范围分别为9.0-45.1μg.mL^-1（r=0.9993）、3.3-16.7μg.mL^-1（r=0.9989）和5.0-25.0μg.mL-1（r=0.9996）。平均回收率分别为：99.5%（RSD=1.04%）、96.3%（RSD=1.37%）和100.8%（RSD=0.81%）。本方法同时测定三种维生素具有快速、简便、准确的特点。

To establish a HPLC method for determination of water-soluble vitamins in drug. A Micropak MCH-10 was used by HPLC and the Column temperature was 40℃. Detector wavelength. 254 nm; flow rate. 1 mL · min^-1. pH was adjusted by phosphate acid. Gradient elution conditions. 20M methanol in water （pH=3） for 3min, and then 40% methanol in water （pH=6） for the rest elution. The liner ranges of vitamin C, vitamin B2 and niacin amide were at 9.0-45.1μg·mL^-1 （r=0.9993）, 3.3- 16.7μg·mL^-1 （r=0. 9989） and 5.0-25.0μg·mL^-1（r=0. 9996）. The average recovery were 99.5% （RSD= 1.04%）, 96.3% （RSD= 1.37%） and 100.8% （RSD= 0. 81%）, respectively. This method is rapid, convenient and accurate for the simultaneous determination of three vitamins.