小鼠子宫内膜异位症模型的建立及其异位内膜蛋白质的差异表达谱分析

Development of an experimental model of endometriosis using mice that differentially express ectopic endometrial proteins

目的:同源子宫内膜异位移植建立小鼠子宫内膜异位症模型,并初步观察异位内膜蛋白质的差异表达。方法:完整剥离小鼠子宫内膜,按每段5 mm分别接种于右侧子宫旁、右侧腹壁和小肠系膜。动物分别于术后30,90和180 d剖腹探察模型成功率,并采用双向电泳初步分析建模180 d异位内膜的蛋白质差异表达。结果:内膜移植30,90和180 d模型的成功率分别为25.0%,50.0%和84.2%;不同接种部位异位内膜形态以清亮囊肿为主,子宫旁内膜移植成功率明显高于另外2个接种部位。双向电泳图谱提示,异位内膜与正常内膜之间完全匹配的蛋白点有99个,丰度大于5倍的差异点有13个,异位子宫内膜的蛋白质表达在种类和数量上明显增多。结论:同源子宫内膜异位移植90和180 d可以成功建立小鼠子宫内膜异位症模型,异位子宫内膜的蛋白质表达明显增多增强。

Objective： To establish a homologous mouse model of endometriosis to analyze the differential expression of the ectopic endometrial proteins. Methods： Homogenous endometrial segments （5 mm each segment） obtained from mice was transplanted onto right lateral uterus, abdominal wall and small intestinal membrane, respectively. The recipient mice were raised for 30, 60 and 90 days, and then were euthanized, respectively. Endometriotic lesions that developed in the uterus, abdomen and intestine were then examined. The differential expression of the proteins in the recipient mice for 180 days was analyzed by 2D electrophoresis. Results： The transplanted ratio of the endometrium was 25.0% , 50.0% and 84.2% , respectively, in the recipient mice for 30, 90 and 180 days. The heterotopic endometrial lesions at different tissues were more clearly existing in clear cyst. Historically, endometriotic lesions at uterus were sharply higher than at the abdomen and intestine. The 2D electrophoresis indicated that the normal endometrium had 99 protein dots that were the same as the endometriotic lesions. The endometriotic lesions showed 13 differential dots that were as 5 times brighter as the normal endometrium in the abundance. The endometriotic lesions expressed more quantity and types of proteins than the normal endometrium. Conclusion： The present endometriosis model using mice would be useful for expeditious quantitative evaluation of extopic endometriotic proteins.