摘要
从水稻绿叶中分离纯化得到一种单亚基的乙醇酸氧化酶 ,其全酶分子量 (Mr)约为 2 60kD ,电泳特性表明该酶的pI <8.3.我们用改进的 8%PAGE ,结合Westernblot,测定水稻绿叶中还存在一种 pI >8.3的全酶Mr>
Glycolate oxidase (GO) was purified from rice green leaves. purified GO showed 260 kD molecular weight, pI<8.3 in 4%～20% PAGE and only one subunit in SDS-PAGE. By using a modified method and 8% PAGE Western blot, molecular weight of GO with high pI(pI>8.3) from crude protein of rice green leaves was assayed to be higher than 669kD.Research showed that GO was very unstable.
出处
《华南师范大学学报（自然科学版）》
CAS
1997年第1期50-54,共5页
Journal of South China Normal University(Natural Science Edition)
基金
中国科学院上海植物生理研究所植物分子遣传国家重点实验室课题!(10 4KF - 92 0 2 )
国家自然科学基金
华南师范大学校内项目基金的
