人工北虫草指纹图谱研究

Study on fingerprint spectrum of artificial Cordyceps militarise

目的:建立人工北虫草TLC和HPLC法指纹图谱。方法:TLC法以三氯甲烷-乙酸乙酯-异丙醇-水(5∶2∶6∶0.6)为展开剂,磷酸氢二钠制备的GF_(254)板展开;HPLC法以乙腈-水梯度洗脱,检测波长260nm。结果:HPLC法精密度、重复性、稳定性试验中共有峰面积、保留时间的RSD均小于3.0%;不同产地的人工北虫草相似度大于0.9。结论:该方法简便可靠,可作为控制人工北虫草质量的依据。

Objective： To constitute fingerprint of artificial Cordyceps militarise. Method： We use chloroform - ethyl acetate - isopropanol - water as developing agent in TLC. While in HPLC, we use acetonitrile and water as mobile phase,gradient elution,the detection wavelength was 260 nm. Result：The RSD values of peak area and retention time of common peaks in precision, repeatability and stability were lower than 3.0% ;respectively, similarity was over 0.9 in artificial Cordyceps militarise collected from different habitats. Conclusion： All results above exhibit that this method is single, practicable, and reliable as a standard method in controlling the quality of artificial Cordyceps militarise