摘要
目的:建立骨健口服液中阿魏酸的RP-HPLC含量测定方法。方法:采用反相高效液相色谱法,色谱拄:ZORBAX E-clipseXDBC18(150×4.6mm,5μm),流动相:乙腈:0.085%(v/v)磷酸水溶液=15:85,检测波长:314nm,流速:1.0mL·min^-1。结果:阿魏酸在1.0~12.0μg·mL^-1范围内呈良好的线性关系(r=0.9998),三个浓度的平均加样回收率分别为99.1%、100.2%、101,2%,RSD分别为2.2%、1.7%、2.1%。结论:该方法简便、准确,可用于控制骨健口服液制剂的质量。
Objective:To establish a method for determination of Ferulic acid in Gujian Oral Solution. Method :The samples were determined by RP-HPLC on a ZORBAX Eclipse XDB C18 column (150 × 4.6mm, 5μm). The mobile phase consisted of acetonitril and 0. 085% (v/v) phostrate acid (15 : 85);the detection wavelength was at 314 nm and the flow rate was 1.0 mL·min^-1. Results:The calibration curves of Ferulic acid was linear in the range of 1.0~12.0μg· mL^-1(r=0. 9998). The average recovery were 99.1%, 100. 2 %, 101.2 % of three different concentrations,and RSD were 2.2%, 1.7 %, 2.1%, respectively. Conclusion .. The method is simple and accurate,which can be used for the quality control of Gujian Oral Solution.
出处
《中国药品标准》
CAS
2007年第1期59-62,共4页
Drug Standards of China