水稻种子萌发过程中降解半乳甘露聚糖的三个酶活性的动态变化

Changes in Activities of Three Enzymes Degrading Galactomannan During and Following Rice Seed Germination

为了研究在种子萌发过程中与细胞壁半乳甘露聚糖降解相关的β-甘露聚糖酶、β-甘露糖苷酶和α-半乳糖苷酶之间的关系,对水稻种子萌发过程中这3种酶的活性变化及GA3和ABA对酶活性的影响进行了研究。在干种子和萌发前的种子中β-甘露糖苷酶和α-半乳糖苷酶活性就已经存在,而β-甘露聚糖酶活性只在萌发后才能检测到;3种酶活性均随着萌发进程而增加;GA3对β-甘露聚糖酶和α-半乳糖苷酶活性具有一定的促进作用。ABA对β-甘露糖苷酶和α-半乳糖苷酶活性的影响不明显,但却可以明显抑制β-甘露聚糖酶活性。

In order to investigate the relationships among β-mannanase,β-mannosidase and α-galactosidase required for degrading galactomannan in cell wall during and following rice seed germination, the activities of the three enzymes and the effects of ABA and GA3 on them were detected. The activities of β-mannosidase and α-galactosidase were detected in dry and non-germination rice seeds, and increased slowly during and following germination. However, the activity of β-mannanase was detected only after germination. GA3 could promote the activities of β-mannanase and α-galactosidase and ABA had little effect on the activities of β-mannanase and α-galactosidase, but inhibited seriously the activity of β-mannanase.