银杏苦内酯B对重症急性胰腺炎大鼠p38丝裂原活化蛋白激酶活性的影响

Effect of ginkgolide B on p38 mitogen-activated protein kinase activity in rat pancreas with severe acute pancreatitis

目的观察银杏苦内酯B(BN52021)对重症急性胰腺炎(SAP)大鼠胰腺组织p38丝裂原活化蛋白激酶(p38MAPK)活性的影响。方法Wistar雄性大鼠随机分为阴性对照组(NC组)、SAP模型组(SAP组)、BN52021治疗组(BN组),每组按术后不同时相点(1、2、3、6、12、24h)分为6小组,用Western blotting法分别检测胰腺组织p38MAPK表达与激活情况,同时对胰腺组织进行病理学观察和测定血清淀粉酶活性的变化。结果血清淀粉酶和病理学结果显示SAP造模成功,BN52021能降低SAP大鼠的血清淀粉酶活性,病理学评分在3、6、12h较SAP组显著降低(P<0.05);NC组在各时相点均只检测到少量磷酸化的p38MAPK,SAP组和BN组在各时相点均较NC组显著升高(P<0.05),BN组在6、12、24h较SAP组显著降低(P<0.05)。结论BN52021可部分抑制SAP大鼠胰腺组织p38MAPK的活化,从而发挥其治疗作用。

Objective To investigate the effects of ginkgolide B （BN52021） on p38 mitogenactivated protein kinase （p38 MAPK） activity in rat pancreas with severe acute pancreatitis （SAP）. Methods One hundred and eighty Wistar male rats were randomly divided into three groups, which included negative contrast group （NC）, SAP model group （SAP） and BN52021 treating group （BN）. The rats of each group were further randomly divided into six subgroups at different time points including 1, 2, 3, 6, 12, and 24 h. The activities of serous amylase were detected in the rats of every subgroups, and the expression and activation of p38 MAPK in pancreas tissue were detected by Western blotting, and the pathologic change of pancreas was observed by microscope after HE dyeing. Results The results of detection of serous amylase and pathology showed that SAP models were successful and BN52021 can depress the activities of serous amylase in SAP group and ameliorate pathological score of pancreas （3, 6, and 12 h, P〈0.05） compared to SAP group. In the rat pancreas of the NC group, the slight phosphorylated p38 MAPK can be detected. Compared with the NC group, the activation of p38 MAPK were significantly higher at every time points in the SAP and BN group （P〈0. 05）. In the BN group, the change of p38 MAPK expression and activation synchronized with that of the SAP group, but at 6, 12, and 24 h points, the level of p38 MAPK was significantly lower （P〈0.05）. Conclusion BN52021 could partly inhibit the activation of p38 MAPK in rat pancreas with SAP and exerts its therapeutic effect.