绵羊支原体肺炎诊断及病原的分离与鉴定

Isolation and Identification of Mycoplasma Ovinpneumoniae

通过对送检病羊的临床症状、剖检变化、病料染色镜检,初步诊断为绵羊支原体肺炎。又经无菌采取病料接种改良Frey培养氏液,再取培养液接种于固体培养液后分离到可疑绵羊肺炎支原体。然后将培养生长的支原体分别进行革兰氏染色、姬姆萨染色、瑞氏染色和碱性复红染色;进行了各种生化试验,进行动物和鸡胚接种试验和平扳凝集等血清学试验;最后确诊为绵羊支原体肺炎,并分离获得了绵羊肺炎支原体。该结果为绵羊支原体肺炎的防制奠定了基础。

Mycoplasma Ovinepneumoniae （MO） could cause severe respiratory diseases of sheep, and make great damages to the sheep husbandry. The pathogeny is isolated from the sheep of severe symptoms and inoculated in advanced Frey and solid medium. Then the culture is dyed through Giemsa and Wrights stain. The plate agglutination is also done, and the result is positive. The culture is inoculated in SPF chicken embryo and rabbits. All the results are positive and show the pathogeny is MO. The morphology, characteristics and property of the MO are generalized. The knowledge provides information for the diagnosis, prophylaxis, the development of specific vaccine and the further research on the monoelonal antibody of the MO.