应用间接免疫荧光技术检测人巨细胞病毒-PP65抗原的临床意义

Detection and its clinical application of human cytomegalovirus PP65 antigen by indirect immunofluorescent assay

目的:探讨人巨细胞病毒(HCMV)-PP65抗原检测的临床意义。方法:利用间接免疫荧光技术对本院56例住院患者外周血HCMV-PP65抗原进行检测,其中器官移植组35例(肾移植患者13例,肝移植患者14例,骨髓移植患者8例),其他疾病组21例。设本院健康体检者20例为正常对照组。结果:56例标本中有25例检出HCMV-PP65抗原阳性,阳性率为45%。25例抗原阳性患者中,有临床症状的患者外周血中的HCMV-PP65抗原阳性细胞数明显高于无临床症状的HCMV-PP65抗原阳性的患者,两者比较差异有显著性(P<0.01)。正常对照组无一例阳性。结论:HCMV-PP65抗原检测具有简便、敏感的特点,其结果与患者的临床症状相关,可以作为监测HCMV活动性感染的指标,为临床提供诊治依据。

Objective To investigate the detecting method and its clinical significance of human cytomegalovirus （HCMV）-PP65 antigen by indirect immunofluorscent assay in hospitalized infection patients. Methods Fifty and six hospitalized patients（13 kidney transplant recipients, 14 liver transplant recipients, 8 medulla transplant recipients and 21 of other diseases） and 20 healthy subjects were taken as control group. CMV-PP65 antigen was defected by indirect immunofluorescent assay. Results HCMV-PP65 were positive in 25 cases （45%） of 56 hospitalized infection patients and were negative in 20 healthy subjects. The numbers of CMV-PP65 positive cells increased significantly in CMV-PP65 positive patients with various clinical symptom than those in the patients with no clinical symptom（P 〈 0.01 ）. Conclusion The detection of CMV-PP65 antigen can identify the patients with early infected HCMV conveniently and sensitively.