摘要
目的改进氟轻松维B6乳膏中维生素B6的含量测定方法。方法流动相由甲醇-水(25∶75)改为甲醇-用冰乙酸调节pH=3.0的0.04%庚烷磺酸钠溶液(25∶75),检测波长由320nm改为291nm。结果维生素B6质量浓度在3.4～17.0μg/mL范围内与峰面积呈良好的线性关系,平均回收率为97.1%,RSD为1.2%(n=6)。结论改进后的方法准确、重现性好、精密度高,可用于测定氟轻松维B6乳膏中维生素B6含量。
Objective To improve the assay method of vitamin B6 in Vitamin B6 and Fluocinonide Cream. Methods To change the mobile phase from methanol- purified water（25：75）to methanol-0. 04% sodium heptanesulfonate solution（adjusting pH to 3.0 with acetic acid, 25 ： 75）, and the detection wavelength from 320 nm to 291 nm. Results The methodological study showed a good linear correlation in the range from 3.4 μg/mL to 17.0 μg/mL. The average recovery rate was 97. 1%, RSD = 1.2% （ n = 5）. Conclusion The improved method is accurate with good reproducibility and high precision. The method can be used for the determination of vitamin B6 in vitamin B6 and fluocinonide cream.
出处
《中国药业》
CAS
2007年第10期42-43,共2页
China Pharmaceuticals
