维甲酸导致胎鼠肾积水的实验研究

Experiment study for tretinoin leads to hydronephrosis of embryorats

目的诱导建立肾积水动物模型,探讨其病理学改变。方法用维甲酸建立胎鼠肾积水模型,观察肾盂输尿管连接部的形态和神经纤维的分布。结果模型组胎鼠肾积水的发生率为63.63%,模型胎鼠肾盂输尿管连接部平滑肌排列紊乱,胶原纤维明显增多;S-100在正常的胎鼠肾盂输尿管连接部组织中呈阳性反应,在肾积水模型组中表达明显减少或呈阴性反应。结论维甲酸制作的先天性肾积水动物模型的病理改变与人类先天性肾积水的病理改变相似,可以作为研究先天性肾积水的动物模型使用。

Objective To establish an hydronephrosis animal model indued with tertinoin in order to explore its pathological change. Methods We used tretinoin （vitamin A acid） induced hydronephrosis of embryorat to establish a animal model to observe the morphology of pyeloureter junctive zone and its distribution of nerve fibers. Results The hydronephrosis morbidity in model group was 63,63%.Arrangeme of smooth muscles of embryorat showed more disorder collagen fibers obviously increased,S-100 protein of embryorat in the pyeloureter junctive zone was significantly decreased,even negative reaction in model group as compared with normal embryorats, Conclusions Animal model of hydronephrosis induced with tretinoin in embryorats have similar pathological changes with human congenital hydronephrosis,it could apply to study the congenital hydronephrosis in animal model.