男性不育患者精液中衣原体、支原体感染的现状研究

STUDY ON THE INFECTION OF UREAPLASMA UREALYTICUM AND CHLAMYDIA TRACHOMATIS IN INFERTILE MEN'S SPERM

[目的]了解泸州地区男性不育者精液中衣原体(CT)、支原体(UU)感染现状,探讨男性感染衣原体、支原体后与不育的关系。[方法]采用荧光定量聚合酶链反应(FQ-PCR)技术对228例男性不育者(不育组)和156例正常生育者(对照组)的精液作CT和UU检测。[结果]不育组CT阳性率为34/228(14.91%),UU阳性率为88/228(38.62%),CT+UU阳性率为22/228(9.65%);对照组CT阳性率为2/156(1.28%),UU阳性率为7/156(4.49%),CT和UU总阳性率为1/156(0.64%)。不育组CT、UU和CT+UU的阳性检出率均明显高于对照组(P﹤0.005)。[结论]男性精液中感染CT、UU是导致不育的重要因素之一,对不育男性应进行CT、UU常规检查并及时治疗非常必要。

[Objective] To study the current situation of infection of ureaplasma urealyticum and Chlamydia trachomatis in infertile man＇s sperm in Luzhou area, and explore the association of Chlamydia trachomatis and Ureaplasma urealyticum infection with infertility. [Methods] Fluorescence quantitative PCR （FQ-PCR） was used to quantitatively detect UU DNA and CT DNA in the sperm of 228 men with infertility and 156 normal men, [Results] In infertility group： CT positive rate was 34/ 228 （14.91%）, UU positive rate was 88/228 （38.62%）, UU＋CT positive rate was 22/228 （9.65%） . In normal men group： CT positive rate was 2/156 （1.28%）, UU positive rate was 7/156 （4.49%）, UU＋CT positive rate was 1/156 （0.64%） . There were significant differences in positive rates of CT, UU AND UU＋CT between the infertile man group and the normal man group （P 〈 0.005） . [Conclusion] CT and UU infection in men＇s sperm was one of the factors causing infertility. It is necessary for the infertile men to receive routine examination and treatment in time.