内皮祖细胞和单个核细胞移植对糖尿病大鼠血管病变疗效的比较

Comparison of transplantation of endothelial progenitor cells and mononuclear cells on the treatment of ischemic disorder in the hind limb of diabetic rats

目的比较内皮祖细胞(EPC)和单个核细胞(MNC)移植改善糖尿病大鼠后肢血管病变的疗效。方法将大鼠EPC和外周血MNC标记后分别植入糖尿病大鼠缺血后肢。对照组仅植入PBS溶液。ELISA法检测VEGF的含量,免疫组化法计数毛细血管数目。结果EPC和MNC均能在缺血后肢分化为毛细血管。移植后EPC组和MNC组大鼠缺血肢体局部VEGF含量高于对照组,毛细血管数目也高于对照组。但EPC组和MNC组VEGF含量和毛细血管数目无明显差异。结论移植EPC及含有相当数量EPC的MNC对糖尿病大鼠后肢血管病变的改善作用相似。EPC可能在外周血单个核细胞移植中发挥主要作用。

Objective To compare the effects of transplantations of endothelial progenitor cells （EPC） and rnononuclear cells （MNC） on improving the ischernic disorder of hind limbs in diabetic rats. Methods The in vitro cultured EPC were transplanted into the ischernic area of 10 diabetic rats in EPC group. 10 diabetic rats in MNC group accepted peripheral MNC transplantation. 8 rats in control group were transplanted with PBS. ELISA method was used to detect the concentration of VEGF in the ischernic area. The numbers of capillary were counted through irnrnunohistochernistry staining. Results EPC and peripheral MNC were able to differentiate into capillaries in the ischernic area. The concentrations of VEGF and the numbers of capillary were higher in both EPC group and MNC group than in control group, with no significant difference between EPC group and MNC group. Conclusions The effects of allotype EPCs and MNC transplantations with equivalent number of EPCs on diabetic rats were similar. EPC may play a major role in peripheral MNC transplantation in treating ischernic disorder.