HPLC测定复方岗稔片中大黄素的含量

Determination of Emodin in Compound Gangren Tablets by HPLC

目的建立HPLC测定复方岗稔片中大黄素含量。方法采用C18柱（4．6mm×250mm，5μm）；流动相：甲醇-0．1％磷酸（85：15）；检测波长：254nm。结果大黄素在4．8—76．8μg／ml范围内，呈良好线性关系（r=0．9999）；平均加样回收率为99．2％，RSD为0．9％。结论该方法准确，灵敏度高，重复性好，可用于复方岗稔片中大黄素的测定。

Objective To establish a HPLC method for determination of emodin in Compound Gangren tablets. Methods The separation was performed on Phenomenex C18 column（ 4.6 mm × 250 mm,5μm ）, the mobile phase was methanol-0.1% phosphoric acid solutions（85：15 ）with detective wavelength at 254 nm. Results The calibration curve for emodin was linear in the range of 4.8 ～ 76.8 μg/ml （ r = 0. 9999 ）, the average recovery was 99.2%, with RSD 0. 9%. Conclusion This method is accurate, sensitive, reproducible, and it can be used for determination of emodin in Compound Gangren tablets.