凋亡抑制基因x-相关细胞凋亡抑制蛋白基因在肝癌细胞中的表达及临床意义

Expression and clinical significance of Antiapoptosis Gene XIAP in liver cancer

目的探讨x-相关细胞凋亡抑制蛋白基因(XIAP)mRNA及蛋白在肝癌组织和癌旁组织中的表达情况,及其与肝癌发生的关系。方法肝癌患者手术切除标本10例,应用半定量逆转录-聚合酶链反应(RT-PCR)分别检测肝癌组织和癌旁组织细胞XIAPmRNA基因的相对表达量,并通过免疫组织化学SP法检测10例肝癌组织和癌旁组织标本XIAP蛋白的表达情况。结果(1)半定量RT-PCR显示:XIAP基因在肝癌组织中均呈高表达,相对表达量为3.211±2.43,而在对应的癌旁组织中呈低表达或无表达,相对表达量为1.947±1.890,两组之间差异有统计学意义(P<0.05)。(2)免疫组织化学及图像分析结果显示:在肝癌组织和癌旁组织中XIAP蛋白平均灰度扫描值分别为161.800±29.470和144.240±26.290,两组之间差异有统计学意义(P<0.01)。结论XIAP在肝癌中的表达明显增高,可能在肝癌的发生、发展过程中发挥重要作用。

Objective To explore the expression of the inhibitor of apoptosis gene XIAP in liver cancer and its clinical significance. Methods Ten cases of human liver cancer specimens were collected from the patients who received operation for liver cancer from Feb 2005 to Jan 2006. Semi-quantitative RTPCR assay and immunohistochemistry were used to detect the XIAPmRNA and XIAP protein expression respectively. Results Semi-quantitative RT-PCR revealed that the expression of XIAPmRNA in the liver cancer specimens and cirrhosis specimens was 3.211 ± 2.430 and 1. 947 ± 1.89 respectively （ P 〈 0.05 ）. immunohistochemistry showed the level of XIAP protein expression of the cancer speciments （ 161. 800 ± 29.47 ） was much significantly higher than that of the cirrhosis specimens （ 144. 240 ± 26. 290 ） （ P 〈 0.01 ）. Conclusion The levels of XIAP were elevated in the human liver cancer specimens and may be involved in the pathogenesis. XIAP is likely to be used as a target in diagonosis of liver cancer.