银耳多糖硫酸酯的合成及抗肿瘤作用的研究

Studies on Synthesis of Sulfated Tremella fuciformis Polysaccharides and Its Inhibition Effect on Tumor

目的合成银耳多糖硫酸酯,考察其体内抗肿瘤活性。方法采用氯磺酸-吡啶法合成银耳多糖硫酸酯,用肿瘤抑制率评价银耳多糖硫酸酯的抑瘤效果。结果合成了3个银耳多糖硫酸酯SⅠ,SⅡ,SⅢ,硫取代度分别为0.89,0.95,0.99,高效液相色谱测定其相对分子质量分别为84000,90000,96000,银耳多糖硫酸酯SⅠ和SⅢ在12mg.kg-1时对小鼠淋巴瘤的抑制率为59.8%和69.9%,与对照组相比,经统计学处理有显著差异。结论银耳多糖硫酸酯为具有较强抗肿瘤活性的新结构多糖。

OBJECTIVE To synthesize a series of sulfated Tremella fuciformis polysaccharides and investigate its anti-tumor effect in vivo. METHODS Chlorosulfonic acid and pyridine were used as ester reagents to produce sulfated Tremellafuciformis polysaccharides. The inhibitory rate on tumor was used to investigate the effect of sulfated Tremellafuciformis polysaccharide. RESULTS Three sulfated polysaccharides named as S Ⅰ ,S Ⅱ and SⅢ with DS 0. 89,0. 95,0.99 were successfully synthesized, their molecular weight were 84 000,90 000 and 96 000 determined by GPC. The inhibitory rates to lymphoma of S Ⅰ and SⅢ at 12 mg·kg^-1 dose were 59. 8% and 69.9%, Compared with control group there were significant differences. CONCLUSION Sulfated Tremella fuciformis polysaccharides are compounds with innovation structure and certain anti-tumor effect.