紫伪翼手参中4个甾醇苷的分离鉴定

Isolation and structural elucidation of four steroidal glycosides from the sea cucumber Pseudocolochirus violaceus

目的研究中国南海紫伪翼手参的化学成分。方法应用多种色谱技术分离,运用波谱分析和化学方法鉴定化合物的结构。结果分离得到4个甾醇苷单体并分别鉴定为:24-甲基-5α-胆甾烷-7,22Z-二烯-3β-O-β- D-吡喃木糖苷(I),24-甲基-5α胆甾烷-7,24(28)-二烯-3β-O-β-D-吡喃木糖苷(Ⅱ),24-甲基-5α-胆甾烷-7-烯- 3β-β-D-吡喃木糖苷(Ⅲ)和24-乙基-5α-胆甾烷-7-烯-3β-O-β-D-吡喃木糖苷(Ⅳ)。结论4个化合物均为首次从海参中获得单体。

Objective To study the chemical constituents of the sea cucumber Pseudocolochirus violaceus collected from the South China Sea. Methods The compounds were isolated and purified by multi-chromatography and their structures were elucidated based on spectral analysis and chemical evidence. Results Four steroidal glycosides were obtained and identified as： 24-methyl-5α-cholesta-7, 22Z-diene-3β-O-β-D-xylopyranoside （ Ⅰ ）, 24-methyl-5α-cholesta-7, 24 （28）-diene-3β-O-β-D-xylopyrano-side （ Ⅱ ）, 24-methyl-5 α-cholesta-7-ene-3β-O-β-D-xylopyranoside （ Ⅲ ） and 24-ethyl-5α-cholesta-7-ene-3β-O-β-D-xylopyranoside （ Ⅳ ）. Conclusion All the compounds are first reported as pure steroidal glycosides isolated from sea cucumbers.