毛蚶多糖的分离纯化和免疫活性测定

Isolation,purification and immunological activity assay of polysaccharide from Arca subcrenata Lischke

目的从毛蚶体内分离纯化得到毛蚶多糖精品(polysaccharide from Arca subcrenata Lischke．简称ASLP),分析其纯度和单糖组成,同时考察免疫活性。方法经除蛋白、DEAE-52和sephadex-G50柱层析得多糖精品。糖醇乙酸酯法测定单糖组成。体外脾淋巴细胞增殖测定ASLP的免疫活性。结果从毛蚶体内提取到一种均一的多糖组分,其单糖组成只有葡萄糖,ASLP能够显著地促进脾淋巴细胞的增殖。结论从毛蚶中分离纯化得到一种均一的多糖组分,具有显著的体外免疫活性。

Objective To isolate and purify a polysaccharide （ASLP） from Arca subcrenata Lischke. Its purity, neutral monosaccharide component and immunological activity in vitro were determined. Methods The proteins were removed by Scrag method. The crude polysaccharide was purified by DEAE-52 anion-exchange chromatography and Sephadex G-50 gel filtration chromatography. The neutral monosaccharide component was determined with alditol acetates method by GC, Lymphocyte proliferation assay was made to investigate the immuno-modulating activity of ASLP. Results and Conclusion The results indicated ASLP was a homogeneous polysaceharide. It was a glucan with molecular weight of 3500Da. It could increase spleen lymphocyte proliferation remarkably. ASLP also showed significantly immunological activity in vitro.