Seprase在上皮性卵巢癌胸腹水中的表达及其临床预后意义

Expression of seprase in effusions from patients with epithelial ovarian carcinoma and its prognostic significance

目的 探讨Seprase在上皮性卵巢癌胸腹水癌细胞中的表达及其临床预后意义。方法 选择郑州大学第一附属医院和挪威奥斯陆大学附属肿瘤医院2001-2003年住院的65例上皮性卵巢癌患者的胸腹水标本75份，采用免疫组化技术检测了65份卵巢癌腹水和10份胸水细胞石蜡包埋组织中Seprase蛋白的表达，并对其中34份新鲜腹水细胞进行免疫印迹分析，同时检测9份良性腹水脱落细胞作为对照。结果 上皮性卵巢癌胸腹水中Seprase蛋白阳性表达率77．33％（58／75），显著高于良性胸腹水的22．22％（2／9），P〈0．01。Seprase蛋白的表达与卵巢癌患者年龄、FIGO分期、组织学分级和组织学类型无关，而与患者预后呈负相关（P〈0．05）。蛋白印迹表明27／34样本中同时检测到170ku Seprase二聚体和97ku单体，1份仪表达170ku Seprase二聚体。结论 Seprase参与了卵巢癌的发生发展，并与患者不良预后有关，可能成为卵巢癌的预后指标。

Objective To explore the expression of seprase in effusions from patients with epithelial ovarian carcinoma and its clinical significance. Metthods The expression pattern of seprase protein was detected in a cohort of seventy-five sections from malignant peritoneal （n =65） and pleural （n = 10） effusions from patients with epithelial ovarian carcinoma by using immunohistochemistry（IHC）. Additionally, thirty-four samples were studied by western blot. Nine reactive effusions, obtained from patients with benign lesions, were also investigated as control. Results Of 75 malignant effusion specimens, 58 （77. 33% ） were positively stained for seprase by IHC, while only 2 of 9 in control group （ P 〈 0. 01 ）. Increased seprase protein was negatively associated with patients＇ overall survival （p 〈 0. 05）. However, there was no significant correlation between protein expression and any clinical pathological factors. Both 170-kD dimeric form and 97-KD monomeric form of seprase were shown in 27 of 34 malignant effusion specimens by western blot, while only one was detected 170-KD dimeric form. Conclusion Seprase is involved in the development of ovarian cancer and its expression in malignant effusions has negative effects on patient＇s outcome, indicating that seprase may be apotential predictive marker.