摘要
目的:探讨p16在脑胶质瘤中的表达及其意义。方法:采用免疫组织化学、核酸原位杂交和多重PCR的方法,检测68例脑胶质瘤和10例正常脑组织标本中p16的表达。结果:p16和mRNA在胶质瘤Ⅰ~Ⅱ级和Ⅲ~Ⅳ级的阳性表达,差异均有统计学意义(P<0.01~P<0.005),而多重PCR检测p16在胶质瘤Ⅰ~Ⅱ级和Ⅲ~Ⅳ级的阳性表达,差异无统计学意义(P>0.05)。结论:p16基因缺失可能与胶质瘤的发生有关;p16低表达在人脑胶质瘤的恶性进展中起重要作用,可作为预后评估指标之一。
Objective:To explore the expression of p16 and its relationship with progression of human brain glioma, Methods:pl6 protein was detected by immunohistochemistry in 68 cases of glioma and 10 cases of normal brain tissues. Nucleic acid hybridization in situ and multiplex polymerase chain reaction (M-PCR) were used to examine the expression of p16 mRNA and DNA in these tissues. Results : Expression of p16 protein and mRNA was higher in glioma grade Ⅰ- Ⅱ than in Ⅲ -Ⅳ( P 〈 0.01 - P 〈 0.00.5 ). By using of M- PCR, the difference in expression of p 16 between glioma grade Ⅰ -Ⅱ and Ⅲ-Ⅳ was not significant (P 〉 0.05 ). Conclusions:Deletion of p16 perhaps had relationship with the occurrence of gliomas;the low expression of p16 may play an important role in progression in human brain gliomas.
出处
《蚌埠医学院学报》
CAS
2007年第3期259-261,共3页
Journal of Bengbu Medical College
基金
安徽省自然科学基金资助项目(00044302)
安徽省优秀青年科技基金资助项目