肺癌患者外周血肺组织特异性基因mRNA检测的诊断价值

Diagnostic value of detection of lung-specific X protein gene mRNA in the peripheral blood of patients with lung cancer using real-time RT-PCR assay

目的：探讨荧光定量RT—PCR法测定外周血肺组织特异性基因（1ung-specific X protein gene，LUNX）mRNA表达对肺癌的诊断价值。方法：荧光定量RT—PCR法检测67例肺癌患者（其中30例手术患者测定术前和术后外周血）、40例良性肺疾病患者、20名健康体检者外周血LUNX mRNA表达，67例肺癌患者同时用放免法测定外周血中CEA、CA125、Cyfra21—1。结果：肺癌患者外周血LUNXmRNA表达阳性率（56．7％）均高于CEA、CA125、Cyfra21—1单项或三者联合检测（分别为16．4％、26．9％、11．9％和32．8％）（P〈0．05～P〈0．005）。荧光定量RT—PCR法测外周血LUNXmRNA表达诊断肺癌的敏感性为56．7％，特异性100％，阳性预测值为100％，阴性预测值为67．4％。结论：荧光定量RT—PCR法测定外周血LUNX mRNA可能成为肺癌基因诊断一项较好的指标。

Objective： To explore the value of lung-specific X protein gene （LUNX） mRNA expression in diagnosis of lung cancer. Methods：Real-time RT-PCR assay was used to detect LUNX mRNA in the peripheral blood of 67 patients with lung cancer,40 patients with benign pulmonary disease and 20 healthy volunteers. The serum levels of CEA, CA125 and Cyfra21-1 of 67 patients with lung cancer were simultaneously measured by radioimmunoassay. Results ： The positive rate of LUNX mRNA expression in the peripheral blood of patients with lung cancer was higher than that of each one or combination of CEA, CA125 and Cyfra21-1 （56.7% and 11.9% , 16.4% ,26.9% ,32.8% ,respectively） （ P 〈 0.05 - P 〈 0. 005 ）, and the sensitivity, specificity, positive predictive value and negative predictive value of LUNX mRNA were 56.7% , 100% , 100% and 67.4% by real-time RT-PCR assay, respectively. Conclusions： LUNX mRNA in peripheral blood detected by Real-Time RT-PCR assay may be a good gene indicator for diagnosis of lung cancer.