高效液相色谱法测定九节龙中岩白菜素的含量

Content Determination of Bergenin in Ardisia pusilla by HPLC

目的：测定九节龙中岩白菜素的含量。方法：高效液相色谱法，Hypersil C18 ODS色谱柱，柱温25℃，波长275nm，流动相：甲醇-水（20：80），流速0．600ml/min；压强1400Pa。结果：岩白菜素质量在0．098～0．591μg范围与峰面积值的线性关系良好（r=0．9998），平均回收率102．0％，RSD：1．4％n=6）；在该条件下，九节龙岩白菜素含量在0．59％以上。结论：在本文条件下，九节龙中岩白菜素的含量几乎与矮地茶相同。

Objective ： To determine the content of bergenin in Ardisia pusilla. Methods ： High Performance Liquid Chromatography was performed on Hyersil C18ODS. The chromatorgraphic conditions were as follows： methanol-water（20： 80） as mobile phase, flow rate being 0. 600 ml/min and detecting wavelength at 275 nm. The column temperature was 25℃. Results ： A good linearity of bergenin was shown in range of 0. 098-0. 591 μg（ r =0. 9998 ）, the average recovery is 102. 0%, RSD = 1.4% （n = 6）. The content of bergenin in Ardisia pusilla is 59% at least. Conclusion ： The content of bergenin in Ardisia pusilla and A. japonica is identical.