肾衰宁分散片治疗腺嘌呤致慢性肾衰竭大鼠的实验研究

Study of the Shenshuaining Dispersible Tablets in Treating Chronic Renal Failure Rats Induced by Adenine

目的:观察肾衰宁分散片对慢性肾衰竭(CRF)大鼠的治疗作用。方法:72只SD大鼠,雌雄各半,随机分为6组:正常对照组、模型对照组、尿毒清组、肾衰宁大、中、小剂量组,后5组大鼠用0.5%腺嘌呤饲料喂养制作CRF模型。各治疗组给相应药物治疗7周后,测定各组大鼠肾功能、血白蛋白、血常规和电解质水平,观察大鼠一般情况和肾脏组织改变。结果:与正常对照组相比,模型组大鼠血清BUN、Scr水平明显升高,白蛋白水平明显降低,钾、钠、磷水平明显升高,钙水平明显降低,红细胞数(RBC)、血红蛋白(HGB)、细胞压积(HCT)水平明显降低,大鼠肾脏指数增大,病理检查出现明显异常;肾衰宁分散片能明显改善上述指标。结论:肾衰宁分散片能通过改善CRF大鼠肾功能,调整血电解质紊乱,升高血清白蛋白水平,改善贫血和肾脏组织病理变化,有效治疗CRF。

Objective： To observe the effect of Shenshuaining dispersible tablets in treating chronic renal failure （CRF） rats. Methods： Seventy-two Sprague-Dawley rats were randomly divided into normal control group, model control group, Niaoduqing group,Shenshuaining dispersible tablets group （high, middle and low dose） . Model of CRF was constnucted by feeding the rats with feed containing O. 5% adenine. After 7 weeks, renal function, albumin （Alb） , blood routine, blood electrolyte were detected and general status, changes of the kidney tissue were observed. Results： Compared with normal rats, the levels of serum blood urea nitrogen （BUN） , creatinine （Cr） were markedly elevated in CRF rats, while the levels of Alb markedly failed. The serum potassium, natrium and phosphorus contents were significantly increased, the serum calcium contents were significantly decreased. The levels of red blood cells （RBC） , hemoglobin and haematocrit were significantly failed. The CRF rats＇ kidney indexes were increased and appeared abnormity under pathology inspection. Shenshuaining dispersible tablets could markedly ameliorate above indexes. Conclusion： Shenshuaining dispersible tablets can treat CRF through effectively ameliorating the CRF rats＇ renal function, regulating the electrolyte turbulence, increasing the levels of Alb and ameliorate anemia and pathological changes of the kidney tissue.