秸秆施用下接种蚯蚓对农田土壤微生物特性的影响

Effects of Earthworm Activities on Soil Microbial Characteristics under Different Straw Amendment Method in Cropland

在连续6年稻麦轮作系统中,研究不同秸秆施用方式下接种蚯蚓对土壤微生物生物量、活性(基础呼吸)及群落功能多样性(BIOLOG单一碳源利用指纹方法)的影响,试验设5个处理:对照(CK)、秸秆表施(M)、秸秆混施(I)、秸秆表施且接种蚯蚓(ME)、秸秆混施且接种蚯蚓(IE)。不同秸秆施用下接种蚯蚓均对土壤微生物生物量、微生物生物活性和群落碳源利用能力产生显著影响:两种秸秆施用方式下接种蚯蚓均增加微生物生物量;秸秆表施并接种蚯蚓导致微生物活性、碳源利用丰富度和多样性指数均降低,而在秸秆混施下则均升高;BIOLOG碳源利用分析结果表明在秸秆施用下接种蚯蚓后土壤的微生物群落组成发生明显变化。

An experiment was conducted to investigate the effects of earthworm activities on soil microbial biomass, activity （basal respiration） and community composition （sole-source carbon utilization, SCU） in a six years rice-wheat rotation system. Five treatments were included： control （CK）, straw mulching （M）, straw incorporation （I）, straw mulching and earthworm inoculation （ME）, straw incorporation and earthworm inoculation （IE）. The presence of earthworms had significant effects on soil microbial biomass, microbial activity and the ability of carbon source utilization by microbe. Microbial biomass increased in the presence of earthworms irrespective of the way of straw application. Soil microbial activity, richness and biodiversity all decreased in the presence of earthworms under straw mulching method, and the reverse was true under straw incorporation method. It was indicated that soil microbial community composition changed significantly under straw application and earthworm activities using BIOLOG carbon utilization analysis.