口腔扁平苔藓中Ki-67表达及与细胞凋亡的关系

Expression of Ki-67 and the relationship with cell apoptosis in oral lichen planus

目的探讨口腔扁平苔藓(OLP)中Ki-67蛋白的表达意义及其与细胞凋亡的关系。方法采用甲基绿-派诺宁染色法及SABC免疫组化方法检测30例OLP及20例正常口腔黏膜(对照组)中Ki-67蛋白表达及细胞凋亡情况。结果OLP中上皮细胞凋亡指数(AI)明显高于正常对照组(t=5.28,P=0.03);OLP中Ki-67蛋白阳性指数显著高于正常对照组(t=5.7374,P=0.001);Ki-67蛋白阳性指数与凋亡指数呈正相关(r=0.8349,P<0.05)。结论OLP中上皮细胞凋亡亢进与Ki-67的异常表达密切相关,可能促进OLP的发生发展。

Objective planus （OLP）, Methods To investigate the expression of Ki-67 and the relationship to apoptosis in oral lichen staining with SABC method and methyl green-pyronine staining were used to detect the expressions of Ki-67 in 30 cases of OLP and 20 cases of normal oral mucosa specimens （the controls）. Results Compared with the controls, OLP was increased in apoptosis index （AI） of epithelial cells （t=5.28, P=0.03）, The expression of Ki-67 in OLP cases was significantly higher than that in the norreal group （t=5.7374, P=0,001）, A positive correlation was observed between Ki-67 expression and AI in the epithelial cells （r=-0,8349, P〈0,05）, Conclusions The accelerated apoptosis of epithelial cells is closely correlated with the expression of Ki-67, which may contribute to the formation and progression of OLP,