摘要
根据巴氏杆菌的链霉素耐药基因StrA和磺胺耐药基因SulⅡ序列设计2对引物,以临床分离的4株禽源巴氏杆菌耐药株为模板,扩增出StrA、SulⅡ基因序列。经克隆和核苷酸序列测定,克隆的基因与文献报道的耐药基因序列(NC-001774)有较高的同源性(≥98.9%);试验结果显示,耐药株巴氏杆菌对链霉素和磺胺耐药性的差异与StrA、SulⅡ基因突变有关(耐药性高的菌株基因突变率高)。试验中所有耐药菌均在StrA基因编码的第204位氨基酸处存在突变;所有耐药菌均在SulⅡ基因编码的第159,246,384,584,590,591,597,582位氨基酸处存在突变,上述突变基因可能是导致巴氏杆菌对链霉素和磺胺产生耐药性的主要因素。
We designed and synthesized a couple of primers according to pastuerella gene bank. The sequences of StrA and Sul Ⅱ were amplified with the 4 pastuerella strains isolated clinically. Cloned gene sequences had high homology as the standard （NC_001774） strain. The result displayed that the differences of streptomycin and sulfonamide drug resistance were related to StrA and Sul Ⅱ gene mutation. All the StrA genes were mutated in 204 and Sul Ⅱ genes was mutated in 159,246,384,584,590,591,597,582 respectively. The mutated genes were main factors that produced streptomycin and sulfonamide resistance in Pastuerella.
出处
《畜牧与兽医》
北大核心
2007年第2期5-7,共3页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金资助项目(30460103)
关键词
巴氏杆菌
StrA基因
SulⅡ基因
基因突变
耐药机制
Pastuerella multocida
StrA gene
Sul Ⅱ gene
gene mutation
the mechanism of drug resistance
