异基因骨髓移植小鼠GVHD模型的建立

A murine model of graft-versus-host disease induced by allogeneic bone marrow transplantation

目的：建立异基因骨髓移植小鼠移植物抗宿主病（GVHD）模型。方法：将供体C57BL／6雄性小鼠骨髓细胞和脾细胞按不同细胞数混合，输入接受照射后的受鼠雌性BALB／c小鼠，观察受鼠的一般情况、生存期、组织病理学和嵌合体检查。结果：给予5×10^6个以上的供者脾细胞的各组小鼠都发生了急性GVHD，但小鼠GVHD出现的时间不同。结论：成功地建立了异基因骨髓移植小鼠GVHD模型，输入5×10^6-5×10^7个供鼠脾细胞均适用于小鼠GVHD防治的研究，可以根据实验需要选择供鼠脾细胞的数量。

Objective：To establish the model of graft-versus-host disease （GVHD） in mice with aliogeneic bone marrow transplantation. Methods ：Bone marrow cells were combined with spleen cells of male donor C57BL/6 mice according to different proportions, then were transfused into female postradiation recipient BALB/c mice. General state, life span and histopathology of the recipient mice and detected chimera were observed. Results and Conclusion： The recipient mice groups which accepted above 5 ×10^6donor spleen cells developed acute GVHD after different peroids of time. The GVHD model in mice after allo-BMT was successfully established. The transfusion of 5 ×10^6-5×10^7 spleen cells may be adequate to establish the murine model of GVHD for the prevention and treatment of GVHD. The number of murine spleen cells can be chosen according to the experimental requirement.