TNF-α对体外培养人牙囊细胞OPG mRNA的影响

Regulation of secretion of OPG mRNA in human dental follicle cells by TNF-α

目的：肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）对体外培养的人牙囊细胞（dental follicle cells,DFC）表达骨保护素（osteoprotegerin,OPG）的影响,探讨TNF-α在牙齿萌出过程中的作用。方法：原代培养人牙囊细胞;取3-6代细胞,培养基中加入TNF-α,在不同浓度和时间点,MTT检测细胞增殖,RT-PCR检测OPG mRNA的表达。结果：TNF-α在10-50 ng/mL促进人牙囊细胞的增殖,TNF-α与牙囊细胞共孵育,轻度降低OPG的mRNA的表达。结论：TNF-α在体外培养的牙囊细胞有降低OPG表达的作用。

AIM： To study the effects of tumor necrosis factor - 1 α （TNF - α） on the expression of osteoprotegerin （OPG） in human dental follicle cells. METHODS： The culture of human dental follicle cells was established , and the third to sixth passage cells were incubated with TNF - α for both time and dose dependent studies. MTT assays were used to evaluate the cells proliferation. Reverse transcriptase- polymerase chain reaction （RT- PCR ） were used to measure the amount of OPG mRNA in the conditioned mediums. RESULTS： This study showed that the proliferation activity increased significantly by dental follicle cells after incubation with 10 - 50 ng/mL TNF - α at third day. The expression of OPG slightly reduced after incubation of the human dental follicle cells with 10 ng/mL TNF - α at 1 h. CONCLUSIONS ： TNF - α can slightly reduced the expression of OPG in human dental follicle cells.