焦点黏附激酶在肝细胞癌组织中表达及其与预后的关系

Focal adhesion kinase mRNA overexpression in hepatocellular carcinoma ( HCC ) and correlation thereof with prognosis of HCC

目的观察焦点黏附激酶(FAK)在不同肝癌组织中表达差异及 FAK 表达在判断肝细胞癌预后中的意义。方法回顾性分析了我所50例肝细胞癌患者临床资料。利用实时 PCR 法检测了肿瘤组织和癌周组织中 FAK mRNA 表达,并分析了 FAK mRNA 表达与肝细胞癌临床病理及预后的关系。结果 FAK mRNA 在肿瘤组织中的表达明显高于癌周组织表达(P<0.001),癌栓组中癌栓中 FAK mRNA 表达高于肿瘤组织表达(P<0.05),癌栓组中肿瘤组织 FAK mRNA 表达明显高于无癌栓组肿瘤组织表达(P=0.003)。高侵袭组中肿瘤组织 FAK mRNA 表达明显高于低侵袭组表达(P=0.020)。单因素与多因素分析发现 FAK 表达是影响肝细胞癌术后生存率的独立因素。结论 FAK在肝细胞癌发生、发展中有重要作用,FAK 表达是肝细胞癌预后指标之一。

Objective To investigate whether focal adhesion kinase （FAK） is involved in the progression of human hepatocellular carcinoma （HCC） and whether FAK mRNA expression has prognostic significance for HCC. Methods Real-time PCR and immunohistochemistry were used to det4ec the mRNA and protein expression of FAK in 50 specimens of HCC obtained during operation. The correlation between FAK expression and clinnicopathologic parameters was. Results The mRNA expression of FAK was significantly higher in the HCC specimens than in the corresponding non-cancerous liver tissues （P 〈 0. 001 ）, in the embolism than in the tumor tissue by the emboli of the same specimen （P 〈 0. 05）, and in the HCC with embolism than in the HCC without embolism （P = 0. 003 ）. Cox regression analysis showed that the FAK mRNA expression was correlated significantly with embolism （P = 0. 003 ） and invasion （ P = 0. 020）. Univariate and multivariate analyses revealed that FAK expression was an independent prognostic factor for survival. Conclusion FAK plays an important role in HCC progression, especially in vascular invasion and FAK expression has prognostic significance for HCC.