针刺对围绝经期大鼠卵巢颗粒细胞凋亡及相关基因表达的影响

Effects of acupuncture on granulocyte apoptosis and expressions of apoptosis-associated genes in the ovary of perimenopausal rats

目的:探讨针刺治疗围绝经期综合征的作用机制。方法:将围绝经期大鼠随机分为针刺组、药物组和围绝经期组,分别进行针刺治疗、更年安药物治疗和空白对照,并与青年大鼠作对照。测定各组大鼠的卵巢颗粒细胞凋亡,检测卵巢颗粒细胞Bcl-2、Fas蛋白表达。结果:与青年大鼠比较,围绝经期大鼠卵巢颗粒细胞凋亡显著增加(P<0·01),Bcl-2蛋白表达明显降低(P<0·01),Fas蛋白表达明显升高(P<0·01)。针刺治疗后围绝经期大鼠卵巢颗粒细胞凋亡减少(P<0·05),Bcl-2蛋白表达提高(P<0·05),Fas蛋白表达明显降低(P<0·01);更年安药物治疗后,卵巢颗粒细胞凋亡亦有降低但图像分析结果无统计学意义(P>0·05),Bcl-2蛋白表达提高(P<0·05),Fas蛋白表达明显降低(P<0·01)。结论:针刺能够抑制围绝经期大鼠卵巢颗粒细胞凋亡,上调卵巢颗粒细胞Bcl-2蛋白表达、下调Fas蛋白表达。

Objective To explore the mechanism of acupuncture for treatment of perimenopausal syndrome. Methods The rats of perimenopausal syndrome were randomly divided into 3 groups, including an acupuncture group treated with acupuncture, a medication group with Gengnian＇an, and a perimenopausal control group, with young rats used for a control group. Granulocyte apoptosis and expressions of Bcl-2 and Fas proteins in the ovary of the rat were detected. Results Granulocyte apoptosis increased significantly （P〈0. 01）, expression of Bcl-2 proteins decreased significantly （P〈0.01） and expression of Fas proteins increased significantly （P〈0.01） in the ovary of perimenopausal rats as compared with the young rats; after acupuncture treatment, granulocyte apoptosis decreased significantly （P〈0.05）, expression of Bcl-2 proteins increased significantly （P〈0.05） and expression of Fas proteins decreased significantly （P〈0. 01） ; after treatment of Gengnian＇an, granulocyte apoptosis did not significantly change （P〉0.05）, expression of Bcl-2 proteins increased significantly （P〈0.05） and expression of Fas proteins decreased significantly （P〈0. 01）. Conclusion Acupuncture can inhibit granulocyte apoptosis, up-regulate expression of Bcl-2 proteins and down-regulate expression of Fas proteins in the ovary of the perimenopausal rat.