满天星试管苗与其玻璃化苗的RAPD指纹图谱分析

Analysis of RAPD Fingerprint of Shoots and Its Vitrification Shoots in vitro of Gypsophila paniculata L.

采用分离群体分组分析法(BSA),用100个随机引物对满天星的正常苗和玻璃化苗进行RAPD分析的结果表明,7个随机引物扩增出多态性差异条带。再用上述7个引物分别对试管苗及其玻璃化苗个体进行DNA的PCR扩增的结果显示,引物J20在2种苗中出现差异条带。

In order to identify vitrification shoots at molecular level, analysis of random amplified polymorphic DNA （RAPD） fingerprinting method was performed on the normal and vitrification shoots in vitro of Gypsophila paniculata. One hundred random primers were amplified products by bulked segregation analysis （BSA）, and seven primers which producted polymorphic bands were screened among them. The seven primers were used on normal and vitrification shoots for PCR amplification, and the result indicated that different bands were amplified by primer J20 in the two shoots.