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甲醇固定导致绿色荧光蛋白的荧光消失 被引量：6

Fluorescence of Green Fluorescent Protein was Disappeared in Cells Fixed with Methanol
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摘要 发现用甲醇固定转染了绿色荧光蛋白(GFP)基因的细胞会导致GFP的荧光消失,而当用聚甲醛固定时,GFP的荧光就没有失去。因此建议避免用甲醇对有GFP表达的细胞进行免疫组化前的固定。 It was found that the fluorescence of GFP in cells transfected with green fluorescent protein （GFP） gene disappeared when those cells were fixed with methanol, whereas those fixed with paraformaldehyde remained fluorescent brightly. Therefore it is suggested that methanol be avoided in fixing cells expressing GFP for immunohistochemistry.
作者 李荣芳 袁慧 刘定干
机构地区 湖南农业大学动物科技学院 中国科学院上海生命科学研究院生物化学与细胞生物学研究所分子生物学国家重点实验室
出处 《细胞生物学杂志》 CSCD 2007年第2期303-304,共2页 Chinese Journal of Cell Biology
关键词 甲醇 绿色荧光蛋白 methanol green fluorescent protein
分类号 Q78 [生物学—分子生物学]
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参考文献1

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同被引文献29

  • 1刘镭,邢达,陈同生,裴沂辉.利用荧光探针DsRed-Mit的细胞凋亡形态学研究[J].激光生物学报,2006,15(3):267-270. 被引量：4
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引证文献6

二级引证文献8

细胞生物学杂志
2007年 第2期

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