环境条件诱导的家蚕滞育系与非滞育系中游离核苷酸的动态变化(英文)

DYNAMIC CHANGES OF FREE NUCLEOTIDES IN THE SILKWORM UNDERGOING EMBRYONIC DIAPAUSE INDUCED BY ENVIRONMENTAL FACTORS

家蚕二化性品种卵的滞育性是由亲代卵胚胎期接受的环境条件决定。在生物体中,ATP和UTP不仅是遗传物质的原料和能量物质,也是重要的信号分子,它们作为神经递质可以激活许多生理过程。本研究利用高压液相色谱(HPLC)检测了家蚕二化性品种大造刚孵化幼虫和终龄幼虫的游离核苷酸的含量。结果表明,预定次代产滞育卵[亲代卵高温(25℃)光照]比预定次代产非滞育卵[亲代卵低温(15℃)黑暗]的刚孵化幼虫整体特别是头部ATP和UTP含量高,并达到显著水平,随着发育到上簇阶段,这种差异显著增加。这些结果暗示,家蚕体内特别是头部游离核苷酸与由环境诱导的家蚕卵滞育性有关,为进一步研究家蚕脑对环境条件的接受、保持的机制提供了一条重要途径。

Free nucleotide contents in newly hatched larvae and final instar larvae were examined in a bivohine race（Daizo） of the silkworm, Bombyx mori, by high pressure liquid chromatography（HPLC）. ATP and UTP were higher in whole bodies especially in newly hatched larvae experienced the regimen of continuous light and warm temperature（25℃）（LW-condition） throughout the embyrogenesis than in those experienced the regimen of continuous darkness and low temperature（15℃）（DL-condition）. ATP and UTP increased rapidly during the wandering stage and their difference was significant between LW-condition and DL-condition. These results indicated that the triphosphate nucleotide contents induced by environmental factors might involve in diapause destination.