前列腺素E1对糖尿病周围神经病变患者谷胱甘肽过氧化物酶活性的影响

Effect of prostaglandin E1 on the activity of serum GSH-Px in patients with diabetic peripheral neuropathy

目的观察前列腺素E1(PGEl)对糖尿病周围神经病变患者血清谷胱甘肽过氧化物酶(GSH-Px)活性的影响,探讨其在糖尿病周围神经病变中的治疗作用。方法将60例糖尿病周围神经病变患者随机分为PGE1治疗组(PGE1组)和灯盏花素对照组(DZ组),各30例。采用周围神经病变诊断评分标准(DNS)评价两组治疗前后效果,并对两组给药前后血清GSH-Px活性进行测定比较。结果(1)PGE1组与DZ组治疗前DNS评分差异无显著性意义(P>0.05)。PGE1组治疗后DNS评分较治疗前显著下降(P<0.05)。DZ组DNS评分治疗前后改变无显著性意义(P>0.05)。两组治疗后DNS评分差异有显著性意义(P<0.05)。(2)PGE1组与DZ组用药前GSH-Px活性分别为(116.07±9.20)μg/mL、(113.00±8.87)μg/mL,治疗后分别为(138.82±9.68)μg/mL、(114.43±9.11)μg/mL。PGE1组用药前后GSH-Px活性变化差异有显著性意义(P<0.01),DZ组用药前后差异无显著性意义(P>0.05)。两组治疗后GSH-Px活性差异有显著性意义(P<0.05)。结论PGE1可以通过提高GSH-Px活性间接影响氧化平衡从而改善糖尿病周同神经细胞功能。

Objective To observe the effect ofprostaglandin E1 （PGE1） on the activity of serum glutathione peroxidase （GSH-Px） in the patients with diabetic peripheral neuropathy （DPN）, to study the effect of PGE1 on the redox state and to explore its mechanism to promote the nerve recovery. Methods Sixty patients with DPN were randomized into the PGE1 group （n=30） and the breviscapine group （DZ, control group, n=30）. After 20-day successive administration, the therapeutic efficacy of the 2 groups was evaluated with Neuropathy Disability Score （DNS） and the activity of serum GSH-Px of the 2 groups before and after therapy were detected and compared. Results Before therapy, the difference in DNS between the 2 groups was not significant （P〉0.05）. The DNS of the PGE1 group decreased obviously after therapy （P〈0.05 vs before therapy）, but the DNS of the DZ group did not change significantly （P〉0.05）. The difference in DNS between the 2 groups after therapy was statistically significant （P〈0.05）. The activity of GSH-Px of PGE 1 and DZ groups before therapy was （116.07±9.20） and （113.00±8.87） ug/mL, respectively and after therapy was （138.82±9.68） and （114.43±9.11） ug/mL, respectively. There was a significant increase in the activity of GSH-Px between pre- and post-therapy in the PGE1 group （P〈0.01）, but no significant difference in the DZ group （P〉0.05）. The activity between the 2 group was significantly different after therapy （P〈0.05）. Conclusion PGE1 may indirectly inhibit the lipid peroxidation by up-regulating the activity of GSH-Px and then improve the function of peripheral nerve-cell in diabetic patients.