应用准弹性激光散射技术测定膝骨性关节炎关节液微粒体的物理特性(英文)

Physical characteristics of synovial fluid nano-particles in knee osteoarthritis detected with quasi-elasticity laser scattering

背景:不同关节疾病关节液中较大颗粒的粒度存在很大差异,据此可初步判断关节疾病的病情。目的:采用准弹性激光散射技术分析关节液微粒体的物理特性与膝骨性关节炎发生与发展的相关关系。设计:以关节液标本为观察对象的对照实验。对象:实验于2004-10/2006-02在暨南大学附属第一医院中心实验室进行。共收集正常关节液41份,不同严重程度膝骨性关节炎患者的关节液58份(其中早期20份,中期23份,晚期15份)。方法:抽取正常人和膝骨性关节炎患者的关节液,利用准弹性激光散射技术测定关节液微粒体的粒度大小及其分布,利用相分析电泳光散射技术测定关节液微粒体的Zeta电位,采用相关分析方法分析微粒体的粒度、Zeta电位与膝骨性关节炎的相关性。主要观察指标:①2组关节液中微粒体的粒度及其分布。②2组关节液中微粒体的Zeta电位及其分布。③关节液中微粒体的粒度与临床诊断的相关性。结果:①膝骨性关节炎关节液微粒体的平均粒度大于正常关节液[(297±84),(63±23)nmP<0.001],且粒度分布范围较正常关节液宽,同时存有大小不一的颗粒。②正常和膝骨性关节炎关节液微粒体的Zeta电位分别为(-21.88±4.36)mV和(-15.84±3.34)mV,两者存在显著差别(P<0.001);表示膝骨性关节炎关节液中微粒体Zeta电位较正常关节液的大。③关节液中微粒体的平均粒径和Zeta电位与关节疾病的临床诊断存在显著的相关性(rp=0.7972,0.6319,P<0.01)。结论:关节液微粒体的粒度大小和Zeta电位与膝骨性关节炎的发生发展具有明显的相关性,能够反映出膝骨性关节炎疾病的严重程度。

BACKGROUND：There is a great difference of grade size of macrobead in various joint diseases; therefore, it can be used to determine state of joint diseases initially. OBJECTIVE： To explore the physical properties of synovial fluid nano-particles and their correlations with the occurrence of knee osteoarthritis （KOA）. DESIGN ： Controlled experimental study based on synovial fluid samples PARTICIPANTS： A total of 99 synovial fluid samples were collected from normal subjects and KOA patients with various KOA severities. Among them, 41 were normal synovial fluids, 58 were KOA. METHODS： Synovial fluid samples from individuals with and without KOA were obtained. Using the technology of quasi-elastic laser scattering, nano-particle size and its distribution were estimated, and the dynamic/static light scattering spectrometric analyzer allowed the measurement of particles Zeta potentials. A correlation analysis between the particle size, Zeta potentials and the onset of KOA was attempted. MAIN OUTCOME MEASURES： ① Grade size and distribution of microsome in synovial fluid; ② Zeta potentials and distribution of microsome in synovial fluid; ③ grade size and clinical correlation of microsome in synovial fluid. RESULTS ： ① The mean nano-particle diameter in the synovial fluid of KOA patients were significantly greater than those of normal joints [（297±84）, （63±23） nm, P 〈 0.001]. The distribution curve of KOA synovial fluid nano-particle size was wider than that of normal fluid. ② The Zeta potentials in the synovial fluid of KOA patients were （-21.88 ±4.36） mV of normal knee and （-15.84 ±3.34） mV of KOA patients, and there was a significant difference （P 〈 0.001）. This suggested that the Zeta potentials in the synovial fluid of KOA patients were significantly greater than those of normal joints. ③ The average particle size and Zeta potential of synovial fluid strongly correlated with the integrity of the joint of KOA （rp =0.797 2, 0.631 9, P〈 0.01）. CONCLUSION： The nano-particle size and Zeta potential of synovial fluid are significantly correlated with the development of KOA, and this can reflect the severity of KOA.