肝细胞增殖因子促进受损肝细胞增殖的可能机制

Mechanisms of augmenter of liver regeneration in promoting damaged hepatocyte proliferation

目的:探讨肝细胞增殖因子(ALR)促进受损肝细胞增殖的可能机制。方法:采用细胞增殖试验(MTT法)观察经ALR刺激的大鼠肝枯否细胞(KC)的条件培养液(KCCM+)对受损肝细胞增殖的影响,同时用免疫组织化学方法检测正常大鼠肝脏组织中ALR的分布、大鼠枯否细胞膜表面结合的ALR以及ALR对枯否细胞IL-6表达的影响。结果:受损肝细胞经枯否细胞条件培养液刺激后增殖明显。正常大鼠肝细胞内可表达合成ALR,枯否细胞膜表面可见ALR免疫反应颗粒,经ALR刺激后枯否细胞IL-6免疫反应信号增强。结论:ALR可能通过首先刺激枯否细胞,从而促进受损肝细胞增殖。

AIM： To investigate the mechanisms of augmenter of liver regeneration （ALR） in promoting damaged hepatocyte proliferation. METHODS ： The effects of Kupffer cell condition medium （ KCCM ＋ ） stimulated by ALR on damaged hepatocyte proliferation were studied by MTT. The localization of ALR binding to Kupffer cell membrane and in intact rat liver was studied by immunohistochemistry. The IL -6 expression in Kupffer cells stimulated with ALR was observed by immunohistochemistry. RESULTS： The proliferation of damaged hepatocytes stimulated with KCCM ＋ was increased significantly. ALR immunostaining particles in plasm of hepatocyte were found in intact liver. The rough immunostaining particles of ALR were seen on the surface of Kupffer cell membrane. Immunostaining particles of IL -6 in Kupffer cells induced by ALR increased. CONCLUSION： ALR promotes proliferation of damaged hepatocytes indirectly by stimulating Kupffer cells.