用基因芯片研究尼古丁对肺腺癌细胞凋亡基因表达的影响

Effect of nicotine in vitro on expressions of apoptosis-related genes in human lung adenocarcinoma cells by cDNA microarray

目的:探讨尼古丁对人肺癌细胞生长的影响及其机制。方法:采用MTT法分析尼古丁处理人肺腺癌细胞SPC-A后生长变化;采用基因芯片技术检测尼古丁处理SPC-A-1细胞前后,细胞凋亡相关基因表达的变化。结果:尼古丁对SPC-A细胞的生长具有显著的促进作用,但随着尼古丁浓度的增大,促进作用也下降。100μg/L尼古丁处理后,SPC-A-1细胞451个凋亡相关基因中有显著变化的基因有80个,其中,促进凋亡相关基因上调的有29个,下调的有13个;抑制凋亡相关基因上调的有26个,下调的有12个。结论:尼古丁能促进人肺腺癌细胞生长,调节许多凋亡相关基因表达。

AIM ： To investigate effect of nicotine on growth of human lung adenocarnoma cells and expressions of apoptosis - related gene. METHODS ： Lung adenocarcinoma cell line, SPC - A - 1, was cultured in the presence of various concentrations （ 1 - 1 000 μg/L） of nicotine for 48 hours. MTT was applied to evaluate effect of nicotine in vitro on growth of SPC - A - 1 cell line. After SPC - A - 1 cells were treated with 100 μg/L for 48 hours, eDNA expression profile microarray was used to detect the expressions of 451 apoptosis -related genes in SPC -A - 1 cell line. RESULTS： Significant proliferation in SPC - A - 1 cells treated with nicotine （ 1 - 10 μg/L） was observed, but this effect decreased with increase in concentration of nicotine in culture. Growth inhibition rate of 1, 10, 100, 1 000μg/L of nicotine was 27%, -40%, -40% and -93%. Mieroarray detection showed that significantly different expressions appeared in 80 of 451 apoptosis - related genes. 29 apoptosis - promoted genes and 26 apoptosis - inhibited genes were up - regulated significantly （ CY3/CY5 〉 2. 0）, and 25 genes were significantly down - regulated （ CY3/CY5 〈 0. 5 ）. CONCLUSION： Nicotine may promote growth of human lung adenocarcinoma cell through regulating many apoptosis - related gene expressions.