正常动脉和动脉粥样硬化动脉内膜上血管细胞黏附因子1的表达和分布

Expression and distribution of vascular cell adhesion molecule-1 in the endarterium of normal artery and atherosclerotic artery

目的:比较正常动脉及糖尿病和非糖尿病患者动脉粥样硬化动脉内膜血管细胞黏附因子1的表达和分布,分析血管细胞黏附因子1在糖尿病动脉粥样硬化进展中的作用。方法:实验于2005-01/2006-05在沈阳医学院及沈洲医院完成。标本分别来源于外伤、肿瘤手术及截肢术者的腹主动脉、股动脉及肾动脉。健康人14例,年龄(36±16)岁,非糖尿病伴动脉粥样硬化患者16例,年龄(63±3)岁,作为非糖尿病组;糖尿病伴动脉粥样硬化患者12例,年龄(64±7)岁,作为糖尿病组。应用免疫组织化学方法检测各组动脉内膜血管细胞黏附因子1的表达和分布,利用计算机图像分析仪对免疫组织化学染色切片进行灰度扫描作相对定量分析。结果:①血管细胞黏附因子1沉积物在正常动脉内膜表达阴性或极少,动脉内膜平均灰度值为132.094±5.180。②在非糖尿病伴动脉硬化患者动脉内膜表达阳性,且散在分布在泡沫细胞、平滑肌细胞周围,动脉内膜平均灰度值为117.561±8.054。③在糖尿病伴动脉硬化患者的动脉内膜表达明显增多,主要分布在内皮和平滑肌细胞周围,中层也可见到血管细胞黏附因子1免疫沉积物表达,内膜增生明显,动脉内膜平均灰度值为84.881±6.119。利用计算机图像分析仪进行灰度扫描,灰度值越小,染色程度越高,即血管细胞黏附因子1的免疫沉积越多。结论:血管细胞黏附因子1在糖尿病患者动脉中表达增加可以部分解释糖尿病患者易于发生动脉粥样硬化。

AIM： To compare the expression and distribution of vascular cell adhesion molecule-1 （VCAM 1） in atherosclerotic plaques from normal persons, diabetic patients and non-diabetic patients, and analyze the effect of VCAM 1 in diabetic atherosclerosis. METHODS： The experiment was performed at the Shenyang Medical College and Shenzhou Hospital from January 2005 to May 2006. The samples were collected from abdominal aorta, femoral artery and renal artery of patients in surgery, tumor operation and amputation surgery, respectively. Totally 14 healthy persons aged （36±16） years, 16 non-diabetic patients with atherosclerosis aged （63 ±3） years as non-diabetic group, 12 diabetic patients with atherosclerosis aged （64±7） years as diabetic group. Immunohistochemical method was performed on arteries to measure the expression and distribution of VCAM 1 in atherosclerotic plaques of each group. Immunohistochemical staining section was judged with computer image scanning to make relatively quantitative analysis. RESULTS： ①VCAM 1 deposition expressed negative or few poli in normal endarterium, with the mean gray value of 132.094±5.180. ②It expressed positive in non-diabetic patients with atherosclerosis, scattered around foam cells and smooth muscle cell, and the mean gray value of endartedum was 117.561±8.054. ③The expression became more significantly in the endarterium of diabetic patients with atherosclerosis, mainly scattered around endothelial tissue and smooth muscle cell. VCAM 1 deposition appeared in stratum medium; the hyperplasy of endomembrane was obvious; the mean gray value of endarterium was 84.881±6.119. Gray scanning was performed with computer image analysis. The smaller the gray value, the higher the staining degree was, and the more the immune deposition of VCAM 1 was. CONCLUSION： The increased expression of VCAM 1 in the artery of diabetic patients can be explained partially for easily onset of atherosclerosis in diabetic patient.